| RRC ID |
3478
|
| 著者 |
Uchino K, Imamura M, Shimizu K, Kanda T, Tamura T.
|
| タイトル |
Germ line transformation of the silkworm, Bombyx mori, using the transposable element Minos.
|
| ジャーナル |
Mol Genet Genomics
|
| Abstract |
We investigated the use of Minos as a vector for transgenesis in the silkworm, Bombyx mori. We first constructed a vector plasmid with the green fluorescent protein (GFP) gene fused with the silkworm cytoplasmic actin gene (A3) promoter, and a helper plasmid with the Minos transposase gene controlled by the same A3 promoter. Injection of the vector and helper plasmid DNA into silkworm eggs produced transgenic animals in the following generation. The efficiency of transgenic silkworm production using this method was much lower than that obtained using piggyBac-mediated germ line transformation. However, >40-fold increase in the efficiency of producing transgenic silkworms was obtained using an in vitro synthesized source of Minos transposase mRNA. We conclude that the Minos transposon is a useful vector for construction of transgenic silkworms, particularly when in vitro synthesized mRNA is used. This is the first report showing that Minos can be used as a vector for germ-line transformation in lepidopteran insects.
|
| 巻・号 |
277(3)
|
| ページ |
213-20
|
| 公開日 |
2007-3-1
|
| DOI |
10.1007/s00438-006-0176-y
|
| PMID |
17216225
|
| MeSH |
Actins / genetics
Animals
Animals, Genetically Modified
Base Sequence
Bombyx / genetics*
DNA Primers / genetics
DNA Transposable Elements / genetics*
Genes, Insect
Genetic Vectors
Green Fluorescent Proteins / genetics
Insect Proteins / genetics
Molecular Sequence Data
Plasmids / genetics
Promoter Regions, Genetic
RNA, Messenger / genetics
Recombinant Proteins / genetics
Transformation, Genetic*
Transposases / genetics*
|
| IF |
2.797
|
| 引用数 |
29
|
|
WOS 分野
|
GENETICS & HEREDITY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| カイコ |
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