RRC ID 3478
Author Uchino K, Imamura M, Shimizu K, Kanda T, Tamura T.
Title Germ line transformation of the silkworm, Bombyx mori, using the transposable element Minos.
Journal Mol. Genet. Genomics
Abstract 40-fold increase in the efficiency of producing transgenic silkworms was obtained using an in vitro synthesized source of Minos transposase mRNA. We conclude that the Minos transposon is a useful vector for construction of transgenic silkworms, particularly when in vitro synthesized mRNA is used. This is the first report showing that Minos can be used as a vector for germ-line transformation in lepidopteran insects.
Volume 277(3)
Pages 213-20
Published 2007-3
DOI 10.1007/s00438-006-0176-y
PMID 17216225
MeSH Actins / genetics Animals Animals, Genetically Modified Base Sequence Bombyx / genetics* DNA Primers / genetics DNA Transposable Elements / genetics* Genes, Insect Genetic Vectors Green Fluorescent Proteins / genetics Insect Proteins / genetics Molecular Sequence Data Plasmids / genetics Promoter Regions, Genetic RNA, Messenger / genetics Recombinant Proteins / genetics Transformation, Genetic* Transposases / genetics*
IF 2.734
Times Cited 26
WOS Category GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Silkworms ?