RRC ID 34802
Author Xu J, Zhang P, Kusakabe T, Mon H, Li Z, Zhu L, Iiyama K, Banno Y, Morokuma D, Lee JM.
Title Comparative proteomic analysis of hemolymph proteins from Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-sensitive or -resistant silkworm strains during infections.
Journal Comp Biochem Physiol Part D Genomics Proteomics
Abstract We reported previously that baculovirus AcMNPV host-ranges in silkworm strains are controlled by a novel third chromosomal locus. To further isolate the potential host factor and uncover the functional pathway involved, in this study we analyzed hemolymph proteins from AcMNPV-resistant or -sensitive silkworm strains infected with baculoviruses. All the protein spots from 2D electrophoresis were characterized by MALDI-TOF MS and further systematically assessed for differentially regulated proteins at different stages of infection. Subsequently, six candidates were selected for functional analysis using Bm5 cells, where the candidates were knocked-down or overexpressed. We observed that mRNA expression levels of beta-N-acetylglucosaminidase and prophenoloxidase subunit 2 are significantly upregulated during AcMNPV infections in Bm5 cells. Ultimately, we found that RNA interference of ribosomal protein RpL34 causes serious damages to cell viability as well as abortive infection, indicating that ribosomal components are essential for productive baculovirus infection.
Volume 16
Pages 36-47
Published 2015-12-1
DOI 10.1016/j.cbd.2015.07.003
PII S1744-117X(15)00051-9
PMID 26276225
MeSH Acetylglucosaminidase / analysis Acetylglucosaminidase / genetics Animals Bombyx / cytology Bombyx / genetics Bombyx / virology* Catechol Oxidase / analysis Catechol Oxidase / genetics Cell Line Enzyme Precursors / analysis Enzyme Precursors / genetics Gene Expression Regulation Hemolymph / metabolism Hemolymph / virology* Host-Pathogen Interactions* Insect Proteins / analysis* Insect Proteins / genetics* Nucleopolyhedroviruses / isolation & purification Nucleopolyhedroviruses / physiology* Proteomics Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
IF 3.011
Times Cited 5
WOS Category GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Silkworms d17 w05