Reference - Detail
| RRC ID | 3481 |
|---|---|
| Author | Zhang P, Aso Y, Jikuya H, Kusakabe T, Lee JM, Kawaguchi Y, Yamamoto K, Banno Y, Fujii H. |
| Title | Proteomic profiling of the silkworm skeletal muscle proteins during larval-pupal metamorphosis. |
| Journal | J Proteome Res |
| Abstract |
The silkworm is a typical holometabolous insect going through drastic morphological changes upon metamorphosis from larvae to pupae. Comprehensive studies focusing on the changes help elucidate understanding of a biogenic mechanism. Here, we report the initial profile of the intersegmental muscle (ISM) proteins of the silkworm during larval-pupal metamorphosis. In total, 258 protein spots were resolved by two-dimensional gel electrophoresis (2-DE). Fifty-seven larval proteins were identified, where 3 proteins were exclusively detected in larval samples. Fifty-four other proteins were common in pupal samples. Of these, 12 proteins belonging to the contractile apparatus, metabolism, regulation, and signal transduction were altered in their contents during the metamorphosis from larvae to pupae. Three pupa-defective proteins were identified as isoforms of troponin I, followed by an immunoblotting validation. This data will be helpful in understanding the biochemistry of an insect ISM. |
| Volume | 6(6) |
| Pages | 2295-303 |
| Published | 2007-6-1 |
| DOI | 10.1021/pr070071y |
| PMID | 17497908 |
| MeSH | Animals Bombyx / growth & development* Bombyx / metabolism Electrophoresis, Gel, Two-Dimensional Larva / chemistry Metamorphosis, Biological* Muscle Proteins / analysis* Muscle, Skeletal / chemistry* Muscle, Skeletal / growth & development Protein Isoforms / analysis Proteomics* Pupa / chemistry |
| IF | 4.074 |
| Times Cited | 18 |
| WOS Category | BIOCHEMICAL RESEARCH METHODS |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Silkworms | p22 |