| RRC ID |
34830
|
| Author |
Tsuyama J, Bunt J, Richards LJ, Iwanari H, Mochizuki Y, Hamakubo T, Shimazaki T, Okano H.
|
| Title |
MicroRNA-153 Regulates the Acquisition of Gliogenic Competence by Neural Stem Cells.
|
| Journal |
Stem Cell Reports
|
| Abstract |
Mammalian neural stem/progenitor cells (NSPCs) sequentially generate neurons and glia during CNS development. Here we identified miRNA-153 (miR-153) as a modulator of the temporal regulation of NSPC differentiation. Overexpression (OE) of miR-153 delayed the onset of astrogliogenesis and maintained NSPCs in an undifferentiated state in vitro and in the developing cortex. The transcription factors nuclear factor I (NFI) A and B, essential regulators of the initiation of gliogenesis, were found to be targets of miR-153. Inhibition of miR-153 in early neurogenic NSPCs induced precocious gliogenesis, whereas NFIA/B overexpression rescued the anti-gliogenic phenotypes induced by miR-153 OE. Our results indicate that miR-mediated fine control of NFIA/B expression is important in the molecular networks that regulate the acquisition of gliogenic competence by NSPCs in the developing CNS.
|
| Volume |
5(3)
|
| Pages |
365-77
|
| Published |
2015-9-8
|
| DOI |
10.1016/j.stemcr.2015.06.006
|
| PII |
S2213-6711(15)00187-3
|
| PMID |
26212661
|
| PMC |
PMC4618452
|
| MeSH |
Animals
Cell Differentiation / physiology*
Cerebral Cortex / cytology
Cerebral Cortex / metabolism*
Mice
MicroRNAs / genetics
MicroRNAs / metabolism*
NFI Transcription Factors / genetics
NFI Transcription Factors / metabolism
Neural Stem Cells / cytology
Neural Stem Cells / metabolism*
Neuroglia / cytology
Neuroglia / metabolism*
|
| IF |
6.032
|
| Times Cited |
22
|
|
WOS Category
|
CELL & TISSUE ENGINEERING
CELL BIOLOGY
|
| Resource |
| DNA material |
pCAGGS-LIF (RDB09182)
pCAG-HIVgp (RDB04394)
pCMV-VSV-G-RSV-Rev (RDB04393) |
