Reference - Detail
|Author||Agawa Y, Sarhan M, Kageyama Y, Akagi K, Takai M, Hashiyama K, Wada T, Handa H, Iwamatsu A, Hirose S, Ueda H.|
|Title||Drosophila Blimp-1 is a transient transcriptional repressor that controls timing of the ecdysone-induced developmental pathway.|
|Journal||Mol. Cell. Biol.|
Regulatory mechanisms controlling the timing of developmental events are crucial for proper development to occur. ftz-f1 is expressed in a temporally regulated manner following pulses of ecdysteroid and this precise expression is necessary for the development of Drosophila melanogaster. To understand how insect hormone ecdysteroids regulate the timing of FTZ-F1 expression, we purified a DNA binding regulator of ftz-f1. Mass spectroscopy analysis revealed this protein to be a fly homolog of mammalian B lymphocyte-induced maturation protein 1 (Blimp-1). Drosophila Blimp-1 (dBlimp-1) is induced directly by 20-hydroxyecdysone, and its product exists during high-ecdysteroid periods and turns over rapidly. Forced expression of dBlimp-1 and RNA interference analysis indicate that dBlimp-1 acts as a repressor and controls the timing of FTZ-F1 expression. Furthermore, its prolonged expression results in delay of pupation timing. These results suggest that the transient transcriptional repressor dBlimp-1 is important for determining developmental timing in the ecdysone-induced pathway.
|MeSH||Animals Binding Sites Cell Extracts Cell Nucleus / drug effects Cell Nucleus / metabolism DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Drosophila Proteins / genetics Drosophila Proteins / isolation & purification Drosophila Proteins / metabolism* Drosophila melanogaster / drug effects* Drosophila melanogaster / embryology* Ecdysone / pharmacology* Embryo, Nonmammalian / drug effects Embryo, Nonmammalian / metabolism Gene Expression Regulation, Developmental / drug effects Promoter Regions, Genetic / genetics Pupa / drug effects RNA, Messenger / genetics RNA, Messenger / metabolism Repressor Proteins / genetics Repressor Proteins / isolation & purification Repressor Proteins / metabolism* Sequence Homology, Amino Acid Thermodynamics Time Factors Transcription Factors / genetics Transcription Factors / metabolism Transcription, Genetic* / drug effects|
|WOS Category||BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY|