| RRC ID |
34862
|
| Author |
Mitchell NC, Tchoubrieva EB, Chahal A, Woods S, Lee A, Lin JI, Parsons L, Jastrzebski K, Poortinga G, Hannan KM, Pearson RB, Hannan RD, Quinn LM.
|
| Title |
S6 Kinase is essential for MYC-dependent rDNA transcription in Drosophila.
|
| Journal |
Cell Signal
|
| Abstract |
Increased rates of ribosome biogenesis and biomass accumulation are fundamental properties of rapidly growing and dividing malignant cells. The MYC oncoprotein drives growth predominantly via its ability to upregulate the ribosome biogenesis program, in particular stimulating the activity of the RNA Polymerase I (Pol I) machinery to increase ribosomal RNA (rRNA) transcription. Although MYC function is known to be highly dependent on the cellular signalling context, the pathways interacting with MYC to regulate transcription of ribosomal genes (rDNA) in vivo in response to growth factor status, nutrient availability and cellular stress are only beginning to be understood. To determine factors critical to MYC-dependent stimulation of rDNA transcription in vivo, we performed a transient expression screen for known oncogenic signalling pathways in Drosophila. Strikingly, from the broad range of pathways tested, we found that ribosomal protein S6 Kinase (S6K) activity, downstream of the TOR pathway, was the only factor rate-limiting for the rapid induction of rDNA transcription due to transiently increased MYC. Further, we demonstrated that one of the mechanism(s) by which MYC and S6K cooperate is through coordinate activation of the essential Pol I transcription initiation factor TIF-1A (RRN 3). As Pol I targeted therapy is now in phase 1 clinical trials in patients with haematological malignancies, including those driven by MYC, these data suggest that therapies dually targeting Pol I transcription and S6K activity may be effective in treating MYC-driven tumours.
|
| Volume |
27(10)
|
| Pages |
2045-53
|
| Published |
2015-10-1
|
| DOI |
10.1016/j.cellsig.2015.07.018
|
| PII |
S0898-6568(15)00215-6
|
| PMID |
26215099
|
| MeSH |
Animals
Cell Nucleolus / enzymology
Cell Nucleolus / ultrastructure
Compound Eye, Arthropod / enzymology
Compound Eye, Arthropod / ultrastructure
DNA, Ribosomal / genetics*
DNA, Ribosomal / metabolism
Drosophila melanogaster / genetics*
Drosophila melanogaster / metabolism
Nuclear Proteins / metabolism
Proto-Oncogene Proteins c-myc / physiology*
Ribosomal Protein S6 Kinases / physiology*
Salivary Glands / enzymology
Salivary Glands / ultrastructure
Transcription Factors / metabolism
Transcription, Genetic*
|
| IF |
3.968
|
| Times Cited |
12
|
|
WOS Category
|
CELL BIOLOGY
|
| Resource |
| Drosophila |
10539R-2 |
