論文 - 詳細
| RRC ID | 34871 |
|---|---|
| 著者 | Zhou B, Lindsay SA, Wasserman SA. |
| タイトル | Alternative NF-κB Isoforms in the Drosophila Neuromuscular Junction and Brain. |
| ジャーナル | PLoS One |
| Abstract |
The Drosophila NF-κB protein Dorsal is expressed at the larval neuromuscular junction, where its expression appears unrelated to known Dorsal functions in embryonic patterning and innate immunity. Using confocal microscopy with domain-specific antisera, we demonstrate that larval muscle expresses only the B isoform of Dorsal, which arises by intron retention. We find that Dorsal B interacts with and stabilizes Cactus at the neuromuscular junction, but exhibits Cactus independent localization and an absence of detectable nuclear translocation. We further find that the Dorsal-related immune factor Dif encodes a B isoform, reflecting a conservation of B domains across a range of insect NF-κB proteins. Carrying out mutagenesis of the Dif locus via a site-specific recombineering approach, we demonstrate that Dif B is the major, if not sole, Dif isoform in the mushroom bodies of the larval brain. The Dorsal and Dif B isoforms thus share a specific association with nervous system tissues as well as an alternative protein structure. |
| 巻・号 | 10(7) |
| ページ | e0132793 |
| 公開日 | 2015-7-13 |
| DOI | 10.1371/journal.pone.0132793 |
| PII | PONE-D-15-21766 |
| PMID | 26167685 |
| PMC | PMC4500392 |
| MeSH | Animals Brain / metabolism* Drosophila Microscopy, Confocal NF-kappa B / metabolism* Neuromuscular Junction / metabolism* |
| IF | 2.74 |
| 引用数 | 5 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 5848R-3 |