| RRC ID |
34901
|
| 著者 |
Andreazza S, Bouleau S, Martin B, Lamouroux A, Ponien P, Papin C, Chélot E, Jacquet E, Rouyer F.
|
| タイトル |
Daytime CLOCK Dephosphorylation Is Controlled by STRIPAK Complexes in Drosophila.
|
| ジャーナル |
Cell Rep
|
| Abstract |
In the Drosophila circadian oscillator, the CLOCK/CYCLE complex activates transcription of period (per) and timeless (tim) in the evening. PER and TIM proteins then repress CLOCK (CLK) activity during the night. The pace of the oscillator depends upon post-translational regulation that affects both positive and negative components of the transcriptional loop. CLK protein is highly phosphorylated and inactive in the morning, whereas hypophosphorylated active forms are present in the evening. How this critical dephosphorylation step is mediated is unclear. We show here that two components of the STRIPAK complex, the CKA regulatory subunit of the PP2A phosphatase and its interacting protein STRIP, promote CLK dephosphorylation during the daytime. In contrast, the WDB regulatory PP2A subunit stabilizes CLK without affecting its phosphorylation state. Inhibition of the PP2A catalytic subunit and CKA downregulation affect daytime CLK similarly, suggesting that STRIPAK complexes are the main PP2A players in producing transcriptionally active hypophosphorylated CLK.
|
| 巻・号 |
11(8)
|
| ページ |
1266-79
|
| 公開日 |
2015-5-26
|
| DOI |
10.1016/j.celrep.2015.04.033
|
| PII |
S2211-1247(15)00434-9
|
| PMID |
25981041
|
| MeSH |
Animals
CLOCK Proteins / metabolism*
Circadian Rhythm / physiology*
Drosophila / metabolism*
Female
Male
Period Circadian Proteins / metabolism*
Phosphorylation
|
| IF |
8.109
|
| 引用数 |
15
|
|
WOS 分野
|
CELL BIOLOGY
|
| リソース情報 |
| ショウジョウバエ |
11526R-1 |
