RRC ID 3491
Author Daimon T, Katsuma S, Kang WK, Shimada T.
Title Functional characterization of chitinase from Cydia pomonella granulovirus.
Journal Arch. Virol.
Abstract Baculovirus chitinases (V-CHIAs) play a crucial role in the terminal liquefaction of virus-infected larvae after death. Although v-chiAs from nucleopolyhedroviruses (NPVs) have been well characterized, little is known about v-chiAs from granuloviruses (GVs). We characterized the v-chiA of Cydia pomonella GV (CpGV) by constructing a recombinant Bombyx mori NPV (BmNPV) in which BmNPV v-chiA was replaced by CpGV v-chiA (103CpGV virus). CpGV v-chiA encoded an approximately 70-kDa chitinase with an exo-type substrate preference. CpGV V-CHIA lacked a C-terminal KDEL endoplasmic reticulum retention motif and was suggested to be a secretory protein. Terminal host liquefaction of B. mori larvae and proper folding of BmNPV-encoded cysteine protease (BmNPV V-CATH) were observed following infection with 103CpGV, indicating that CpGV v-chiA is able to compensate for the absence of its BmNPV counterpart. Our data suggest that the molecular interaction between V-CHIA and V-CATH may be conserved across a broad range of lepidopteran GVs and NPVs.
Volume 152(9)
Pages 1655-64
Published 2007
DOI 10.1007/s00705-007-1000-7
PMID 17557135
MeSH Animals Cell Line Chitinases / genetics* Cysteine Endopeptidases / genetics DNA, Viral / genetics Genes, Viral* Granulovirus / enzymology* Granulovirus / genetics* Larva / virology Moths / virology*
IF 2.16
Times Cited 6
WOS Category VIROLOGY
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