| RRC ID |
3491
|
| 著者 |
Daimon T, Katsuma S, Kang WK, Shimada T.
|
| タイトル |
Functional characterization of chitinase from Cydia pomonella granulovirus.
|
| ジャーナル |
Arch Virol
|
| Abstract |
Baculovirus chitinases (V-CHIAs) play a crucial role in the terminal liquefaction of virus-infected larvae after death. Although v-chiAs from nucleopolyhedroviruses (NPVs) have been well characterized, little is known about v-chiAs from granuloviruses (GVs). We characterized the v-chiA of Cydia pomonella GV (CpGV) by constructing a recombinant Bombyx mori NPV (BmNPV) in which BmNPV v-chiA was replaced by CpGV v-chiA (103CpGV virus). CpGV v-chiA encoded an approximately 70-kDa chitinase with an exo-type substrate preference. CpGV V-CHIA lacked a C-terminal KDEL endoplasmic reticulum retention motif and was suggested to be a secretory protein. Terminal host liquefaction of B. mori larvae and proper folding of BmNPV-encoded cysteine protease (BmNPV V-CATH) were observed following infection with 103CpGV, indicating that CpGV v-chiA is able to compensate for the absence of its BmNPV counterpart. Our data suggest that the molecular interaction between V-CHIA and V-CATH may be conserved across a broad range of lepidopteran GVs and NPVs.
|
| 巻・号 |
152(9)
|
| ページ |
1655-64
|
| 公開日 |
2007-1-1
|
| DOI |
10.1007/s00705-007-1000-7
|
| PMID |
17557135
|
| MeSH |
Animals
Cell Line
Chitinases / genetics*
Cysteine Endopeptidases / genetics
DNA, Viral / genetics
Genes, Viral*
Granulovirus / enzymology*
Granulovirus / genetics*
Larva / virology
Moths / virology*
|
| IF |
2.243
|
| 引用数 |
7
|
|
WOS 分野
|
VIROLOGY
|
| リソース情報 |
| カイコ |
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