論文 - 詳細
| RRC ID | 3494 |
|---|---|
| 著者 | Abe H, Fujii T, Tanaka N, Yokoyama T, Kakehashi H, Ajimura M, Mita K, Banno Y, Yasukochi Y, Oshiki T, Nenoi M, Ishikawa T, Shimada T. |
| タイトル | Identification of the female-determining region of the W chromosome in Bombyx mori. |
| ジャーナル | Genetica |
| Abstract |
The W chromosome of the silkworm Bombyx mori is devoid of functional genes, except for the putative female-determining gene (Fem). To localize Fem, we investigated the presence of W-specific DNA markers on strains in which an autosomal fragment containing dominant marker genes was attached to the W chromosome. We produced new W-chromosomal fragments from the existing Zebra-W strain (T(W;3)Ze chromosome) by X-irradiation, and then carried out deletion mapping of these and sex-limited yellow cocoon strains (T(W;2)Y-Chu, -Abe and -Ban types) from different Japanese stock centers. Of 12 RAPD markers identified in the normal W chromosomes of most silkworm strains in Japan, the newly irradiated W(B-YL-YS)Ze chromosome contained three, the T(W;2)Y-Chu chromosome contained six, and the T(W;2)Y-Abe and -Ban chromosomes contained only one (W-Rikishi). To investigate the ability of the reduced W-chromosome translocation fragments to form heterochromatin bodies, which are found in nuclei of normal adult female sucking stomachs, we examined cells of the normal type p50 strain and the T(W;2)Y-Chu and -Abe strains. A single sex heterochromatin body was found in nuclei of p50 females, whereas we detected only small sex heterochromatin bodies in the T(W;2)Y-Chu strain and no sex heterochromatin body in the T(W;2)Y-Abe strain. Since adult females of all strains were normal and fertile, we conclude that only extremely limited region, containing the W-Rikishi RAPD sequence of the W chromosome, is required to determine femaleness. Based on a comparison of the normal W-chromosome and 7 translocation and W-deletion strains we present a map of Fem relative to the 12 W-specific RAPD markers. |
| 巻・号 | 133(3) |
| ページ | 269-82 |
| 公開日 | 2008-7-1 |
| DOI | 10.1007/s10709-007-9210-1 |
| PMID | 17901928 |
| MeSH | Animals Bombyx / genetics* Chromosome Breakage / radiation effects Chromosomes, Artificial, Bacterial / genetics Female Genetic Markers / genetics Male Meiosis / genetics Polymerase Chain Reaction Random Amplified Polymorphic DNA Technique Sex Chromosomes / genetics* Sex Determination Processes* X-Rays |
| IF | 1.186 |
| 引用数 | 30 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カイコ | p50 |