RRC ID 3510
Author Bao Y, Yamano Y, Morishima I.
Title Beta-1,3-glucan inducible expression of prophenoloxidase-activating proteinase from eri-silkworm, Samia cynthia ricini.
Journal Comp. Biochem. Physiol. B, Biochem. Mol. Biol.
Abstract A cDNA clone encoding possible prophenoloxidase-activating serine protease (PAP) was isolated by screening the cDNA library from immunized larval fat body of the wild silkmoth, Samia cynthia ricini. The cDNA encodes a 438 amino acid open reading frame with a predicted 20 residue signal peptide. Samia PAP has high sequence similarity to Bombyx mori and Manduca sexta PAPs, which contain two amino terminal clip domains followed by a carboxyl-terminal catalytic domain. The expression of the gene was barely detectable in the fat body of naive larvae, but induced after injection of the larvae with beta-1,3-glucans or bacterial cells.
Volume 147(1)
Pages 45-8
Published 2007-5
DOI 10.1016/j.cbpb.2006.12.016
PII S1096-4959(07)00010-3
PMID 17307003
MeSH Amino Acid Sequence Animals Base Sequence Bombyx / enzymology* DNA, Complementary / genetics DNA, Complementary / isolation & purification Enzyme Induction / drug effects Molecular Sequence Data Sequence Analysis, DNA Sequence Homology, Amino Acid Serine Endopeptidases / biosynthesis* Serine Endopeptidases / chemistry Serine Endopeptidases / genetics* beta-Glucans / pharmacology*
IF 1.99
Times Cited 5
Silkworms Samia cynthia ricini