RRC ID 3511
著者 Onoe H, Matsumoto A, Hashimoto K, Yamano Y, Morishima I.
タイトル Peptidoglycan recognition protein (PGRP) from eri-silkworm, Samia cynthia ricini; protein purification and induction of the gene expression.
ジャーナル Comp Biochem Physiol B Biochem Mol Biol
Abstract Peptidoglycan recognition protein (PGRP) was isolated from immunized hemolymph of the wild silkworm, Samia cynthia ricini, detecting the biding activity with (125)I-labeled peptidoglycan (PGN). The binding specificity of PGRP was tested by competitive inhibition of the binding to (125)I-labeled-PGN by a large excess amount of non-labeled-PGN or other glucans. The binding to labeled uncross-linked Lys-type PGN from Micrococcus luteus was strongly inhibited by non-labeled-PGN of the same structure and meso-diaminopimelic acid (DAP)-type cross-linked PGN from Bacillus cell wall, but only a little by cross-linked PGN from M. luteus cell wall. The PGRP cDNA encodes a 193 amino acid open reading frame. The deduced amino acid sequence had 62 to 91% identities to known lepidopteran PGRPs, but less than 40% to Drosophila PGRPs. The PGRP gene constitutively expressed at a low level in naive fat body, and strongly induced by an injection of DAP-type cross-linked and Lys-type uncross-linked PGNs, but only weakly by Lys-type cross-linked PGN from M. luteus. The silkworm possibly distinguish between PGNs based on the structure of cross-linking peptide, but has less if any preference for the diamino acid residue of the stem peptide.
巻・号 147(3)
ページ 512-9
公開日 2007-7-1
DOI 10.1016/j.cbpb.2007.03.005
PII S1096-4959(07)00128-5
PMID 17434328
MeSH Amino Acid Sequence Animals Bacillus / chemistry Carrier Proteins / biosynthesis* Carrier Proteins / chemistry Carrier Proteins / genetics* Carrier Proteins / isolation & purification Drosophila / genetics Drosophila / metabolism Gene Expression Regulation / physiology* Insect Proteins / biosynthesis* Insect Proteins / chemistry Insect Proteins / genetics* Insect Proteins / isolation & purification Micrococcus luteus / chemistry Molecular Sequence Data Moths / genetics* Moths / metabolism* Peptidoglycan / chemistry Protein Binding / physiology Sequence Homology, Amino Acid
IF 2.219
引用数 14
WOS 分野 ZOOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
カイコ Samia cynthia ricini