論文 - 詳細
| RRC ID | 3511 |
|---|---|
| 著者 | Onoe H, Matsumoto A, Hashimoto K, Yamano Y, Morishima I. |
| タイトル | Peptidoglycan recognition protein (PGRP) from eri-silkworm, Samia cynthia ricini; protein purification and induction of the gene expression. |
| ジャーナル | Comp Biochem Physiol B Biochem Mol Biol |
| Abstract |
Peptidoglycan recognition protein (PGRP) was isolated from immunized hemolymph of the wild silkworm, Samia cynthia ricini, detecting the biding activity with (125)I-labeled peptidoglycan (PGN). The binding specificity of PGRP was tested by competitive inhibition of the binding to (125)I-labeled-PGN by a large excess amount of non-labeled-PGN or other glucans. The binding to labeled uncross-linked Lys-type PGN from Micrococcus luteus was strongly inhibited by non-labeled-PGN of the same structure and meso-diaminopimelic acid (DAP)-type cross-linked PGN from Bacillus cell wall, but only a little by cross-linked PGN from M. luteus cell wall. The PGRP cDNA encodes a 193 amino acid open reading frame. The deduced amino acid sequence had 62 to 91% identities to known lepidopteran PGRPs, but less than 40% to Drosophila PGRPs. The PGRP gene constitutively expressed at a low level in naive fat body, and strongly induced by an injection of DAP-type cross-linked and Lys-type uncross-linked PGNs, but only weakly by Lys-type cross-linked PGN from M. luteus. The silkworm possibly distinguish between PGNs based on the structure of cross-linking peptide, but has less if any preference for the diamino acid residue of the stem peptide. |
| 巻・号 | 147(3) |
| ページ | 512-9 |
| 公開日 | 2007-7-1 |
| DOI | 10.1016/j.cbpb.2007.03.005 |
| PII | S1096-4959(07)00128-5 |
| PMID | 17434328 |
| MeSH | Amino Acid Sequence Animals Bacillus / chemistry Carrier Proteins / biosynthesis* Carrier Proteins / chemistry Carrier Proteins / genetics* Carrier Proteins / isolation & purification Drosophila / genetics Drosophila / metabolism Gene Expression Regulation / physiology* Insect Proteins / biosynthesis* Insect Proteins / chemistry Insect Proteins / genetics* Insect Proteins / isolation & purification Micrococcus luteus / chemistry Molecular Sequence Data Moths / genetics* Moths / metabolism* Peptidoglycan / chemistry Protein Binding / physiology Sequence Homology, Amino Acid |
| IF | 2.219 |
| 引用数 | 14 |
| WOS 分野 | ZOOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| カイコ | Samia cynthia ricini |