| RRC ID |
3511
|
| 著者 |
Onoe H, Matsumoto A, Hashimoto K, Yamano Y, Morishima I.
|
| タイトル |
Peptidoglycan recognition protein (PGRP) from eri-silkworm, Samia cynthia ricini; protein purification and induction of the gene expression.
|
| ジャーナル |
Comp Biochem Physiol B Biochem Mol Biol
|
| Abstract |
Peptidoglycan recognition protein (PGRP) was isolated from immunized hemolymph of the wild silkworm, Samia cynthia ricini, detecting the biding activity with (125)I-labeled peptidoglycan (PGN). The binding specificity of PGRP was tested by competitive inhibition of the binding to (125)I-labeled-PGN by a large excess amount of non-labeled-PGN or other glucans. The binding to labeled uncross-linked Lys-type PGN from Micrococcus luteus was strongly inhibited by non-labeled-PGN of the same structure and meso-diaminopimelic acid (DAP)-type cross-linked PGN from Bacillus cell wall, but only a little by cross-linked PGN from M. luteus cell wall. The PGRP cDNA encodes a 193 amino acid open reading frame. The deduced amino acid sequence had 62 to 91% identities to known lepidopteran PGRPs, but less than 40% to Drosophila PGRPs. The PGRP gene constitutively expressed at a low level in naive fat body, and strongly induced by an injection of DAP-type cross-linked and Lys-type uncross-linked PGNs, but only weakly by Lys-type cross-linked PGN from M. luteus. The silkworm possibly distinguish between PGNs based on the structure of cross-linking peptide, but has less if any preference for the diamino acid residue of the stem peptide.
|
| 巻・号 |
147(3)
|
| ページ |
512-9
|
| 公開日 |
2007-7-1
|
| DOI |
10.1016/j.cbpb.2007.03.005
|
| PII |
S1096-4959(07)00128-5
|
| PMID |
17434328
|
| MeSH |
Amino Acid Sequence
Animals
Bacillus / chemistry
Carrier Proteins / biosynthesis*
Carrier Proteins / chemistry
Carrier Proteins / genetics*
Carrier Proteins / isolation & purification
Drosophila / genetics
Drosophila / metabolism
Gene Expression Regulation / physiology*
Insect Proteins / biosynthesis*
Insect Proteins / chemistry
Insect Proteins / genetics*
Insect Proteins / isolation & purification
Micrococcus luteus / chemistry
Molecular Sequence Data
Moths / genetics*
Moths / metabolism*
Peptidoglycan / chemistry
Protein Binding / physiology
Sequence Homology, Amino Acid
|
| IF |
2.219
|
| 引用数 |
14
|
|
WOS 分野
|
ZOOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| カイコ |
Samia cynthia ricini |
