RRC ID |
35124
|
Author |
Kubo T, Takashi T, Ashikari M, Yoshimura A, Kurata N.
|
Title |
Two Tightly Linked Genes at the hsa1 Locus Cause Both F1 and F2 Hybrid Sterility in Rice.
|
Journal |
Mol Plant
|
Abstract |
Molecular mechanisms of hybrid breakdown associated with sterility (F2 sterility) are poorly understood as compared with those of F1 hybrid sterility. Previously, we characterized three unlinked epistatic loci, hybrid sterility-a1 (hsa1), hsa2, and hsa3, responsible for the F2 sterility in a cross between Oryza sativa ssp. indica and japonica. In this study, we identified that the hsa1 locus contains two interacting genes, HSA1a and HSA1b, within a 30-kb region. HSA1a-j (japonica allele) encodes a highly conserved plant-specific domain of unknown function protein (DUF1618), whereas the indica allele (HSA1a-i(s)) has two deletion mutations that cause disruption of domain structure. The second gene, HSA1b-i(s), encodes an uncharacterized protein with some similarity to a nucleotide-binding protein. Homozygous introgression of indica HSA1a-i(s)-HSA1b-i(s) alleles into japonica showed female gamete abortion at an early mitotic stage. The fact that the recombinant haplotype HSA1a-j-HSA1b-i(s) caused semi-sterility in the heterozygous state with the HSA1a-i(s)-HSA1b-i(s) haplotype suggests that variation in the hsa1 locus is a possible cause of the wide-spectrum sterility barriers seen in F1 hybrids and successive generations in rice. We propose a simple genetic model to explain how a single causal mechanism can drive both F1 and F2 hybrid sterility.
|
Volume |
9(2)
|
Pages |
221-232
|
Published |
2016-2-1
|
DOI |
10.1016/j.molp.2015.09.014
|
PII |
S1674-2052(15)00391-3
|
PMID |
26455463
|
MeSH |
Amino Acid Sequence
Chromosomes, Plant / genetics
Chromosomes, Plant / metabolism
Germ Cells, Plant / metabolism
Hybridization, Genetic
Molecular Sequence Data
Oryza / genetics*
Oryza / physiology
Plant Infertility*
Plant Proteins / chemistry
Plant Proteins / genetics*
Plant Proteins / metabolism
Sequence Alignment
|
IF |
12.084
|
Times Cited |
28
|
WOS Category
|
PLANT SCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
Resource |
Rice |
W1724
W1945
W1236
W0120
W2103
W1230
W1540
W2109
W2078
W0600
W1087
W1169
W1187
W0137
W1865
W2264
W1962
W2263
W0630
W1681
W0593
W1685
W1635
W1508
W1866
W1551
W1807
W1294
W0652
W0698
W1588
W1921
W2003
W1625
W1214
W1238
W2051
W0610 |