| RRC ID |
35125
|
| Author |
Masuko T, Sugahara K, Kozono M, Otsuki S, Akiyama T, Yamamoto T, Toyoshima K, Hashimoto Y.
|
| Title |
A murine monoclonal antibody that recognizes an extracellular domain of the human c-erbB-2 protooncogene product.
|
| Journal |
Jpn J Cancer Res
|
| Abstract |
A murine IgM monoclonal antibody, designated SV2-61, was generated against human c-erbB-2 gene-transfected NIH-3T3 (SV11) cells. SV2-61 defined a 185-kDa molecule present on the surface of SV11 cells, another line of c-erbB-2 gene-transfected NIH-3T3 (A4-15) cells, and MKN-7 human gastric cancer cell line carrying an amplified human c-erbB-2 gene. The SV2-61-defined antigen was found to show protein kinase activity in vitro. The SV2-61 was reactive with human c-erbB-2 gene-transfected NIH-3T3 cell lines but not with transfectants carrying c-erbB-2 gene mutants which lack a coding region for the extracellular domain. It was reactive with a portion of human epithelial cell lines but not with native NIH-3T3, TGF-alpha-coding gene-, activated c-raf gene- or Ha-ras gene-transfected NIH-3T3 cells, or non-epithelial human cells. These results indicate that the SV2-61 is an antibody which recognizes an extracellular domain of the c-erbB-2 gene product, 185-kDa protein.
|
| Volume |
80(1)
|
| Pages |
10-4
|
| Published |
1989-1-1
|
| DOI |
10.1111/j.1349-7006.1989.tb02237.x
|
| PMID |
2565325
|
| PMC |
PMC5917689
|
| MeSH |
Animals
Antibodies, Monoclonal* / immunology
ErbB Receptors / analysis
Humans
Mice
Mice, Inbred BALB C
Phosphorylation
Proto-Oncogene Proteins / analysis*
Proto-Oncogene Proteins / immunology
Receptor, ErbB-2
Transfection
Tumor Cells, Cultured
|
| Times Cited |
49
|
|
WOS Category
|
ONCOLOGY
|
| Resource |
| DNA material |
pSV2 erb B2 (RDB01273) |
