Reference - Detail
| RRC ID | 35125 |
|---|---|
| Author | Masuko T, Sugahara K, Kozono M, Otsuki S, Akiyama T, Yamamoto T, Toyoshima K, Hashimoto Y. |
| Title | A murine monoclonal antibody that recognizes an extracellular domain of the human c-erbB-2 protooncogene product. |
| Journal | Jpn J Cancer Res |
| Abstract |
A murine IgM monoclonal antibody, designated SV2-61, was generated against human c-erbB-2 gene-transfected NIH-3T3 (SV11) cells. SV2-61 defined a 185-kDa molecule present on the surface of SV11 cells, another line of c-erbB-2 gene-transfected NIH-3T3 (A4-15) cells, and MKN-7 human gastric cancer cell line carrying an amplified human c-erbB-2 gene. The SV2-61-defined antigen was found to show protein kinase activity in vitro. The SV2-61 was reactive with human c-erbB-2 gene-transfected NIH-3T3 cell lines but not with transfectants carrying c-erbB-2 gene mutants which lack a coding region for the extracellular domain. It was reactive with a portion of human epithelial cell lines but not with native NIH-3T3, TGF-alpha-coding gene-, activated c-raf gene- or Ha-ras gene-transfected NIH-3T3 cells, or non-epithelial human cells. These results indicate that the SV2-61 is an antibody which recognizes an extracellular domain of the c-erbB-2 gene product, 185-kDa protein. |
| Volume | 80(1) |
| Pages | 10-4 |
| Published | 1989-1-1 |
| DOI | 10.1111/j.1349-7006.1989.tb02237.x |
| PMID | 2565325 |
| PMC | PMC5917689 |
| MeSH | Animals Antibodies, Monoclonal* / immunology ErbB Receptors / analysis Humans Mice Mice, Inbred BALB C Phosphorylation Proto-Oncogene Proteins / analysis* Proto-Oncogene Proteins / immunology Receptor, ErbB-2 Transfection Tumor Cells, Cultured |
| Times Cited | 49 |
| WOS Category | ONCOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 39 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pSV2 erb B2 (RDB01273) |