RRC ID 35131
著者 Sugiyama M, Akase SP, Nakanishi R, Horie H, Kaneko Y, Harashima S.
タイトル Nuclear localization of Haa1, which is linked to its phosphorylation status, mediates lactic acid tolerance in Saccharomyces cerevisiae.
ジャーナル Appl Environ Microbiol
Abstract Improvement of the lactic acid resistance of the yeast Saccharomyces cerevisiae is important for the application of the yeast in industrial production of lactic acid from renewable resources. However, we still do not know the precise mechanisms of the lactic acid adaptation response in yeast and, consequently, lack effective approaches for improving its lactic acid tolerance. To enhance our understanding of the adaptation response, we screened for S. cerevisiae genes that confer enhanced lactic acid resistance when present in multiple copies and identified the transcriptional factor Haa1 as conferring resistance to toxic levels of lactic acid when overexpressed. The enhanced tolerance probably results from increased expression of its target genes. When cells that expressed Haa1 only from the endogenous promoter were exposed to lactic acid stress, the main subcellular localization of Haa1 changed from the cytoplasm to the nucleus within 5 min. This nuclear accumulation induced upregulation of the Haa1 target genes YGP1, GPG1, and SPI1, while the degree of Haa1 phosphorylation observed under lactic acid-free conditions decreased. Disruption of the exportin gene MSN5 led to accumulation of Haa1 in the nucleus even when no lactic acid was present. Since Msn5 was reported to interact with Haa1 and preferentially exports phosphorylated cargo proteins, our results suggest that regulation of the subcellular localization of Haa1, together with alteration of its phosphorylation status, mediates the adaptation to lactic acid stress in yeast.
巻・号 80(11)
ページ 3488-95
公開日 2014-6-1
DOI 10.1128/AEM.04241-13
PII AEM.04241-13
PMID 24682296
PMC PMC4018848
MeSH Adaptation, Physiological Cell Nucleus / chemistry* Drug Tolerance* Gene Expression Lactic Acid / toxicity* Phosphorylation Protein Processing, Post-Translational Saccharomyces cerevisiae / chemistry* Saccharomyces cerevisiae / drug effects* Saccharomyces cerevisiae Proteins / analysis* Saccharomyces cerevisiae Proteins / genetics Transcription Factors / analysis* Transcription Factors / genetics
IF 4.016
引用数 14
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
酵母 BY23849(BY4741) BY23850(BY4742)