論文 - 詳細
| RRC ID | 35140 |
|---|---|
| 著者 | Ueda Y, Ikushima S, Sugiyama M, Matoba R, Kaneko Y, Matsubara K, Harashima S. |
| タイトル | Large-scale genome reorganization in Saccharomyces cerevisiae through combinatorial loss of mini-chromosomes. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
A highly efficient technique, termed PCR-mediated chromosome splitting (PCS), was used to create cells containing a variety of genomic constitutions in a haploid strain of Saccharomyces cerevisiae. Using PCS, we constructed two haploid strains, ZN92 and SH6484, that carry multiple mini-chromosomes. In strain ZN92, chromosomes IV and XI were split into 16 derivative chromosomes, seven of which had no known essential genes. Strain SH6484 was constructed to have 14 mini-chromosomes carrying only non-essential genes by splitting chromosomes I, II, III, VIII, XI, XIII, XIV, XV, and XVI. Both strains were cultured under defined nutrient conditions and analyzed for combinatorial loss of mini-chromosomes. During culture, cells with various combinations of mini-chromosomes arose, indicating that genomic reorganization could be achieved by splitting chromosomes to generate mini-chromosomes followed by their combinatorial loss. We found that although non-essential mini-chromosomes were lost in various combinations in ZN92, one mini-chromosome (18kb) that harbored 12 genes was not lost. This finding suggests that the loss of some combination of these 12 non-essential genes might result in synthetic lethality. We also found examples of genome-wide amplifications induced by mini-chromosome loss. In SH6484, the mitochondrial genome, as well as the copy number of genomic regions not contained in the mini-chromosomes, was specifically amplified. We conclude that PCS allows for genomic reorganization, in terms of both combinations of mini-chromosomes and gene dosage, and we suggest that PCS could be useful for the efficient production of desired compounds by generating yeast strains with optimized genomic constitutions. |
| 巻・号 | 113(6) |
| ページ | 675-82 |
| 公開日 | 2012-6-1 |
| DOI | 10.1016/j.jbiosc.2012.01.013 |
| PII | S1389-1723(12)00054-0 |
| PMID | 22382015 |
| MeSH | Bioengineering Chromosome Deletion* Chromosomes, Fungal / genetics* Genome, Fungal* Haploidy Polymerase Chain Reaction / methods Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae / ultrastructure |
| IF | 2.366 |
| 引用数 | 10 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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