Reference - Detail
| RRC ID | 35141 |
|---|---|
| Author | Park AH, Sugiyama M, Harashima S, Kim YH. |
| Title | Creation of an ethanol-tolerant yeast strain by genome reconstruction based on chromosome splitting technology. |
| Journal | J Microbiol Biotechnol |
| Abstract |
We sought to breed an industrially useful yeast strain, specifically an ethanol-tolerant yeast strain that would be optimal for ethanol production, using a novel breeding method, called genome reconstruction, based on chromosome splitting technology. To induce genome reconstruction, Saccharomyces cerevisiae strain SH6310, which contains 31 chromosomes including 12 artificial mini-chromosomes, was continuously cultivated in YPD medium containing 6% to 10% ethanol for 33 days. The 12 mini-chromosomes can be randomly or specifically lost because they do not contain any genes that are essential under high-level ethanol conditions. The strains selected by inducing genome reconstruction grew about ten times more than SH6310 in 8% ethanol. To determine the effect of minichromosome loss on the ethanol tolerance phenotype, PCR and Southern hybridization were performed to detect the remaining mini-chromosomes. These analyses revealed the loss of mini-chromosomes no. 11 and no. 12. Mini-chromosome no. 11 contains ten genes (YKL225W, PAU16, YKL223W, YKL222C, MCH2, FRE2, COS9, SRY1, JEN1, URA1) and no. 12 contains fifteen genes (YHL050C, YKL050W-A, YHL049C, YHL048C-A, COS8, YHLComega1, ARN2, YHL046W-A, PAU13, YHL045W, YHL044W, ECM34, YHL042W, YHL041W, ARN1). We assumed that the loss of these genes resulted in the ethanol-tolerant phenotype and expect that this genome reconstruction method will be a feasible new alternative for strain improvement. |
| Volume | 22(2) |
| Pages | 184-9 |
| Published | 2012-2-1 |
| DOI | 10.4014/jmb.1109.09046 |
| PII | JMB022-02-05 |
| PMID | 22370347 |
| MeSH | Chromosomes, Fungal* Drug Tolerance Ethanol / metabolism* Ethanol / toxicity* Gene Deletion Genetics, Microbial / methods* Industrial Microbiology / methods Metabolic Engineering / methods* Molecular Biology / methods Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae / metabolism* |
| IF | 1.992 |
| Times Cited | 5 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Yeast | On hold |