| RRC ID |
35144
|
| 著者 |
Benjaphokee S, Hasegawa D, Yokota D, Asvarak T, Auesukaree C, Sugiyama M, Kaneko Y, Boonchird C, Harashima S.
|
| タイトル |
Highly efficient bioethanol production by a Saccharomyces cerevisiae strain with multiple stress tolerance to high temperature, acid and ethanol.
|
| ジャーナル |
N Biotechnol
|
| Abstract |
Use of super strains exhibiting tolerance to high temperature, acidity and ethanol is a promising way to make ethanol production economically feasible. We describe here the breeding and performance of such a multiple-tolerant strain of Saccharomyces cerevisiae generated by a spore-to-cell hybridization technique without recombinant DNA technology. A heterothallic strain showing a high-temperature (41°C) tolerant (Htg(+)) phenotype, a derivative from a strain isolated from nature, was crossed with a homothallic strain displaying high-ethanol productivity (Hep(+)), a stock culture at the Thailand Institute of Scientific and Technological Research. The resultant hybrid TJ14 displayed ability to rapidly utilize glucose, and produced ethanol (46.6g/l) from 10% glucose fermentation medium at high temperature (41°C). Not only ethanol productivity at 41°C but also acid tolerance (Acd(+)) was improved in TJ14 as compared with its parental strains, enabling TJ14 to grow in liquid medium even at pH 3. TJ14 maintained high ethanol productivity (46.0g/l) from 10% glucose when fermentation was done under multiple-stress conditions (41°C and pH 3.5). Furthermore, when TJ14 was subjected to a repeated-batch fermentation scheme, the growth and ethanol production of TJ14 were maintained at excellent levels over ten cycles of fermentation. Thus, the multiple-stress (Htg(+) Hep(+) Acd(+)) resistant strain TJ14 should be useful for cost-effective bioethanol production under high-temperature and acidic conditions.
|
| 巻・号 |
29(3)
|
| ページ |
379-86
|
| 公開日 |
2012-2-15
|
| DOI |
10.1016/j.nbt.2011.07.002
|
| PII |
S1871-6784(11)00159-2
|
| PMID |
21820088
|
| MeSH |
Biofuels*
Ethanol / metabolism*
Glucose / metabolism*
Hot Temperature
Hydrogen-Ion Concentration
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / growth & development*
Selection, Genetic*
Stress, Physiological / physiology*
|
| IF |
4.674
|
| 引用数 |
59
|
|
WOS 分野
|
BIOCHEMICAL RESEARCH METHODS
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 酵母 |
NA |
