RRC ID 35173
Author Leow SN, Luu CD, Hairul Nizam MH, Mok PL, Ruhaslizan R, Wong HS, Wan Abdul Halim WH, Ng MH, Ruszymah BH, Chowdhury SR, Bastion ML, Then KY.
Title Safety and Efficacy of Human Wharton's Jelly-Derived Mesenchymal Stem Cells Therapy for Retinal Degeneration.
Journal PLoS ONE
Abstract PURPOSE:To investigate the safety and efficacy of subretinal injection of human Wharton's Jelly-derived mesenchymal stem cells (hWJ-MSCs) on retinal structure and function in Royal College of Surgeons (RCS) rats.
METHODS:RCS rats were divided into 2 groups: hWJ-MSCs treated group (n = 8) and placebo control group (n = 8). In the treatment group, hWJ-MSCs from healthy donors were injected into the subretinal space in one eye of each rat at day 21. Control group received saline injection of the same volume. Additional 3 animals were injected with nanogold-labelled stem cells for in vivo tracking of cells localisation using a micro-computed tomography (microCT). Retinal function was assessed by electroretinography (ERG) 3 days before the injection and repeated at days 15, 30 and 70 after the injection. Eyes were collected at day 70 for histology, cellular and molecular studies.
RESULTS:No retinal tumor formation was detected by histology during the study period. MicroCT scans showed that hWJ-MSCs stayed localised in the eye with no systemic migration. Transmission electron microscopy showed that nanogold-labelled cells were located within the subretinal space. Histology showed preservation of the outer nuclear layer (ONL) in the treated group but not in the control group. However, there were no significant differences in the ERG responses between the groups. Confocal microscopy showed evidence of hWJ-MSCs expressing markers for photoreceptor, Müller cells and bipolar cells.
CONCLUSIONS:Subretinal injection of hWJ-MSCs delay the loss of the ONL in RCS rats. hWJ-MSCs appears to be safe and has potential to differentiate into retinal-like cells. The potential of this cell-based therapy for the treatment of retinal dystrophies warrants further studies.
Volume 10(6)
Pages e0128973
Published 2016-6-24
DOI 10.1371/journal.pone.0128973
PII PONE-D-15-01434
PMID 26107378
PMC PMC4479609
MeSH Animals Biomarkers / metabolism Electroretinography Gene Expression Gold / chemistry Humans Injections, Intraocular Mesenchymal Stem Cells / cytology Mesenchymal Stem Cells / physiology* Metal Nanoparticles / chemistry Rats Retina / metabolism Retina / pathology* Retinal Degeneration / metabolism Retinal Degeneration / pathology Retinal Degeneration / therapy* Staining and Labeling / methods Stem Cell Transplantation* Transplantation, Heterologous Wharton Jelly / cytology Wharton Jelly / physiology X-Ray Microtomography
IF 2.766
Times Cited 10
Rats RCS/Kyo (strainID=27)