論文 - 詳細
| RRC ID | 35193 |
|---|---|
| 著者 | Gaur R, Björk GR, Tuck S, Varshney U. |
| タイトル | Diet-dependent depletion of queuosine in tRNAs in Caenorhabditis elegans does not lead to a developmental block. |
| ジャーナル | J Biosci |
| Abstract |
Queuosine (Q), a hypermodified nucleoside,occurs at the wobble position of transfer RNAs (tRNAs)with GUN anticodons. In eubacteria, absence of Q affects messenger RNA (mRNA) translation and reduces the virulence of certain pathogenic strains. In animal cells,changes in the abundance of Q have been shown to correlate with diverse phenomena including stress tolerance, cell proliferation and tumour growth but the function of Q in animals is poorly understood. Animals are thought to obtain Q (or its analogues) as a micronutrient from dietary sources such as gut micro flora. However,the difficulty of maintaining animals under bacteria-free conditions on Q-deficient diets has severely hampered the study of Q metabolism and function in animals. In this study,we show that as in higher animals, tRNAs in the nematode Caenorhabditis elegans are modified by Q and its sugar derivatives. When the worms were fed on Q-deficient Escherichia coli, Q modification was absent from the worm tRNAs suggesting that C.elegans lacks a de novo pathway of Q biosynthesis. The inherent advantages of C.elegans as a model organism, and the simplicity of conferring a Q-deficient phenotype on it make it an ideal system to investigate the function of Q modification in tRNA. |
| 巻・号 | 32(4) |
| ページ | 747-54 |
| 公開日 | 2007-6-1 |
| DOI | 10.1007/s12038-007-0074-4 |
| PMID | 17762147 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Chromatography, High Pressure Liquid Diet* Mass Spectrometry Nucleoside Q / metabolism* RNA, Transfer / isolation & purification RNA, Transfer / metabolism* |
| IF | 1.645 |
| 引用数 | 9 |
| WOS 分野 | BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | |