Reference - Detail
| RRC ID | 35212 |
|---|---|
| Author | Namba A, Mano N, Takano H, Beppu T, Ueda K, Hirose H. |
| Title | OmpA is an adhesion factor of Aeromonas veronii, an optimistic pathogen that habituates in carp intestinal tract. |
| Journal | J Appl Microbiol |
| Abstract |
AIMS:In the present study, we focused on one of the Aeromonas veronii isolates that exhibited marked adhesion onto carp intestine and studied its membrane-associated proteins for their possible involvement in mucosal adhesion. METHODS AND RESULTS:We isolated a strain of Aer. veronii (CWP11) that exhibited a high degree of temperature-dependent adhesion activity onto carp intestinal tract and studied its adhesion factor. A proteomic analysis of the membrane-associated fraction showed the presence of multiple proteins that were specifically expressed in CWP11 cells cultured at 25 degrees C. Of these, a 30 kDa protein was identified to be OmpA by a mass fingerprint analysis. Cloning and nucleotide sequencing of the ompA region of CWP11 revealed the presence of two tandem ompA homologues (ompAI-ompAII). Escherichia coli that expressed either OmpAI or OmpAII exhibited marked adhesion onto carp intestinal surface. Disruption of ompAI by a homologous recombination technique resulted in marked reduction of the adhesion activity in CWP11. CONCLUSION:The OmpA homologue plays an important role in the adhesion of the Aer. veronii strain onto the surface of intestinal tract. SIGNIFICANCE AND IMPACT OF THE STUDY:We successfully identified an OmpA homologue to be an adhesion factor of Aer. veronii, an optimistic pathogen that habituates in carp intestinal tract. |
| Volume | 105(5) |
| Pages | 1441-51 |
| Published | 2008-11-1 |
| DOI | 10.1111/j.1365-2672.2008.03883.x |
| PII | JAM3883 |
| PMID | 18828791 |
| MeSH | Aeromonas / genetics Aeromonas / physiology* Animals Bacterial Adhesion / genetics Bacterial Adhesion / physiology* Bacterial Outer Membrane Proteins* / genetics Carps / microbiology* DNA, Bacterial / genetics Escherichia coli / genetics Fish Diseases / microbiology Intestinal Mucosa / microbiology* Proteome Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA |
| IF | 3.066 |
| Times Cited | 18 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| Resource | |
| Prokaryotes E. coli | CV-43(JM109(Lambdapir)) CV-62(S17-1(Lambdapir)) |