Reference - Detail
| RRC ID | 35227 |
|---|---|
| Author | Liu YS, Zhang YQ, Yang LX, Yao TJ, Xiao CL. |
| Title | Gene-specific silencing induced by parallel complementary RNA in Pseudomonas aeruginosa. |
| Journal | Biotechnol Lett |
| Abstract |
To investigate whether parallel complementary RNA (pRNA) could induce gene-specific silencing in Pseudomonas aeruginosa, pRNA of the mexA gene was expressed in it. Compared to the control strains, the strain expressing pRNA of mexA showed a 50% decrease in minimum inhibitory concentrations (MICs) of several antimicrobial agents and a twofold increase in the initial accumulation rate of ethidium bromide, all of which are substrates of the MexAB-OprM efflux pump. These results suggest that gene-specific silencing was induced by pRNA. This is the first time that such a route for gene silencing has been reported in a bacterium other than Escherichia coli. Gene-specific silencing induced by pRNA may be useful as a novel biotechnology tool for gene regulation in prokaryotes. |
| Volume | 31(10) |
| Pages | 1571-5 |
| Published | 2009-10-1 |
| DOI | 10.1007/s10529-009-0046-x |
| PMID | 19543856 |
| MeSH | Anti-Bacterial Agents / pharmacology Bacterial Outer Membrane Proteins / antagonists & inhibitors Gene Knockdown Techniques / methods* Gene Silencing* Membrane Transport Proteins Microbial Sensitivity Tests Pseudomonas aeruginosa / genetics* RNA, Complementary / genetics* |
| IF | 1.977 |
| Times Cited | 5 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| Resource | |
| Prokaryotes E. coli | |