論文 - 詳細
| RRC ID | 35232 |
|---|---|
| 著者 | Newton GJ, Mori S, Nakamura R, Hashimoto K, Watanabe K. |
| タイトル | Analyses of current-generating mechanisms of Shewanella loihica PV-4 and Shewanella oneidensis MR-1 in microbial fuel cells. |
| ジャーナル | Appl Environ Microbiol |
| Abstract |
Although members of the genus Shewanella have common features (e.g., the presence of decaheme c-type cytochromes [c-cyts]), they are widely variable in genetic and physiological features. The present study compared the current-generating ability of S. loihica PV-4 in microbial fuel cells (MFCs) with that of well-characterized S. oneidensis MR-1 and examined the roles of c-cyts in extracellular electron transfer. We found that strains PV-4 and MR-1 exhibited notable differences in current-generating mechanisms. While the MR-1 MFCs maintained a constant current density over time, the PV-4 MFCs continued to increase in current density and finally surpassed the MR-1 MFCs. Coulombic efficiencies reached 26% in the PV-4 MFC but 16% in the MR-1 MFCs. Although both organisms produced quinone-like compounds, anode exchange experiments showed that anode-attached cells of PV-4 produced sevenfold more current than planktonic cells in the same chamber, while planktonic cells of MR-1 produced twice the current of the anode-attached cells. Examination of the genome sequence indicated that PV-4 has more c-cyt genes in the metal reductase-containing locus than MR-1. Mutational analysis revealed that PV-4 relied predominantly on a homologue of the decaheme c-cyt MtrC in MR-1 for current generation, even though it also possesses two homologues of the decaheme c-cyt OmcA in MR-1. These results suggest that current generation in a PV-4 MFC is in large part accomplished by anode-attached cells, in which the MtrC homologue constitutes the main path of electrons toward the anode. |
| 巻・号 | 75(24) |
| ページ | 7674-81 |
| 公開日 | 2009-12-1 |
| DOI | 10.1128/AEM.01142-09 |
| PII | AEM.01142-09 |
| PMID | 19837834 |
| PMC | PMC2794086 |
| MeSH | Bioelectric Energy Sources* Cytochrome c Group / genetics Cytochrome c Group / metabolism Electricity Electron Transport / genetics Oxidation-Reduction Shewanella / classification Shewanella / genetics Shewanella / physiology* |
| IF | 4.016 |
| 引用数 | 96 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | CV-43(JM109(Lambdapir)) |