RRC ID 35244
著者 Lambert MA, Smith SG.
タイトル The PagN protein mediates invasion via interaction with proteoglycan.
ジャーナル FEMS Microbiol Lett
Abstract Heparan sulphate proteoglycans are major components of the mammalian cell membrane. Here we show that PagN of Salmonella enterica serovar Typhimurium utilizes heparinated proteoglycan to successfully invade mammalian cells. Mutants defective in the production of the outer membrane protein PagN displayed similar levels of invasiveness of glycosylation-deficient pgsA-745 cells in comparison with wild-type Salmonella. Furthermore, pgsA-745 cells were invaded c. 400-fold less efficiently than CHO-K1 cells by Escherichia coli expressing PagN. PagN is likely to interact with heparinated proteoglycan as heparin could inhibit PagN-mediated invasion in a dose-dependent manner. Finally, we show, by deletion analysis, that all four extracellular loops of PagN are crucial for invasion of mammalian cells.
巻・号 297(2)
ページ 209-16
公開日 2009-8-1
DOI 10.1111/j.1574-6968.2009.01666.x
PII FML1666
PMID 19552707
MeSH Amino Acid Sequence Animals CHO Cells Cricetinae Cricetulus Humans Molecular Sequence Data Protein Binding Proteoglycans / metabolism* Salmonella Infections / metabolism* Salmonella Infections / microbiology Salmonella typhimurium / chemistry Salmonella typhimurium / genetics Salmonella typhimurium / metabolism*
IF 1.987
引用数 26
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌)