Reference - Detail
| RRC ID | 35263 |
|---|---|
| Author | Galli E, Gerdes K. |
| Title | Spatial resolution of two bacterial cell division proteins: ZapA recruits ZapB to the inner face of the Z-ring. |
| Journal | Mol Microbiol |
| Abstract |
FtsZ, the essential regulator of bacterial cell division, is a dynamic cytoskeletal protein that forms helices that condense into the Z-ring prior to division. Two small coiled-coil proteins, ZapA and ZapB, are both recruited early to the Z-ring. We show here that ZapB is recruited to the Z-ring by ZapA. A direct interaction between ZapA and ZapB is supported by bacterial two-hybrid and in vitro interaction assays. Using high-resolution 3-D reconstruction microscopy, we find that, surprisingly, ZapB is located inside the Z-ring in virtually all cells investigated. We propose a molecular model in which ZapA increases lateral interactions between FtsZ proto-filaments and ZapB mediates further stabilization of this interaction by cross-linking ZapA molecules bound to adjacent FtsZ proto-filaments. Gene deletion and complementation assays show that ZapB can mitigate cell division and Z-ring assembly defects even in the absence of ZapA, raising the possibility that ZapB stimulates Z-ring assembly by two different mechanisms. |
| Volume | 76(6) |
| Pages | 1514-26 |
| Published | 2010-6-1 |
| DOI | 10.1111/j.1365-2958.2010.07183.x |
| PII | MMI7183 |
| PMID | 20487275 |
| MeSH | Bacterial Proteins / analysis* Carrier Proteins / analysis* Cell Cycle Proteins / analysis* Cell Division* Cytoskeletal Proteins / analysis* Escherichia coli / chemistry* Escherichia coli / physiology* Escherichia coli Proteins / analysis* Gene Deletion Genetic Complementation Test Imaging, Three-Dimensional Microscopy Models, Biological Protein Binding |
| IF | 3.418 |
| Times Cited | 72 |
| WOS Category | MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | |