RRC ID 35282
Author Serventi F, Ramazzina I, Lamberto I, Puggioni V, Gatti R, Percudani R.
Title Chemical basis of nitrogen recovery through the ureide pathway: formation and hydrolysis of S-ureidoglycine in plants and bacteria.
Journal ACS Chem Biol
Abstract While some organisms, including humans, eliminate oxidized purines to get rid of excess nitrogen, for many others the recovery of the purine ring nitrogen is vital. In the so-called ureide pathway, nitrogen is released as ammonia from allantoate through a series of reactions starting with allantoate amidohydrolase (AAH), a manganese-dependent enzyme found in plants and bacteria. We report NMR evidence that the true product of the AAH reaction is S-ureidoglycine, a nonstandard alpha-amino acid that spontaneously releases ammonia in vitro. Using gene proximity and logical genome analysis, we identified a candidate gene (ylbA) for S-ureidoglycine metabolism. The proteins encoded by Escherichia coli and Arabidopsis thaliana genes catalyze the manganese-dependent release of ammonia through hydrolysis of S-ureidoglycine. Hydrolysis then inverts the configuration and yields S-ureidoglycolate. S-Ureidoglycine aminohydrolase (UGHY) is cytosolic in bacteria, whereas in plants it is localized, like allantoate amidohydrolase, in the endoplasmic reticulum. These findings strengthen the basis for the known sensitivity of the ureide pathway to Mn availability and suggest a further rationale for the active transport of Mn in the endoplasmic reticulum of plant cells.
Volume 5(2)
Pages 203-14
Published 2010-2-19
DOI 10.1021/cb900248n
PMID 20038185
MeSH Amino Acid Sequence Aminohydrolases / chemistry Aminohydrolases / genetics Aminohydrolases / metabolism* Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Endoplasmic Reticulum / enzymology Escherichia coli / enzymology* Escherichia coli / genetics Escherichia coli Proteins / chemistry Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Glycine / analogs & derivatives* Glycine / metabolism Hydrolysis Manganese / metabolism* Molecular Sequence Data Nitrogen / metabolism* Protein Structure, Secondary Urea / analogs & derivatives* Urea / metabolism Ureohydrolases / metabolism*
IF 4.374
Times Cited 33
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Prokaryotes E. coli NA