論文 - 詳細
| RRC ID | 35282 |
|---|---|
| 著者 | Serventi F, Ramazzina I, Lamberto I, Puggioni V, Gatti R, Percudani R. |
| タイトル | Chemical basis of nitrogen recovery through the ureide pathway: formation and hydrolysis of S-ureidoglycine in plants and bacteria. |
| ジャーナル | ACS Chem Biol |
| Abstract |
While some organisms, including humans, eliminate oxidized purines to get rid of excess nitrogen, for many others the recovery of the purine ring nitrogen is vital. In the so-called ureide pathway, nitrogen is released as ammonia from allantoate through a series of reactions starting with allantoate amidohydrolase (AAH), a manganese-dependent enzyme found in plants and bacteria. We report NMR evidence that the true product of the AAH reaction is S-ureidoglycine, a nonstandard alpha-amino acid that spontaneously releases ammonia in vitro. Using gene proximity and logical genome analysis, we identified a candidate gene (ylbA) for S-ureidoglycine metabolism. The proteins encoded by Escherichia coli and Arabidopsis thaliana genes catalyze the manganese-dependent release of ammonia through hydrolysis of S-ureidoglycine. Hydrolysis then inverts the configuration and yields S-ureidoglycolate. S-Ureidoglycine aminohydrolase (UGHY) is cytosolic in bacteria, whereas in plants it is localized, like allantoate amidohydrolase, in the endoplasmic reticulum. These findings strengthen the basis for the known sensitivity of the ureide pathway to Mn availability and suggest a further rationale for the active transport of Mn in the endoplasmic reticulum of plant cells. |
| 巻・号 | 5(2) |
| ページ | 203-14 |
| 公開日 | 2010-2-19 |
| DOI | 10.1021/cb900248n |
| PMID | 20038185 |
| MeSH | Amino Acid Sequence Aminohydrolases / chemistry Aminohydrolases / genetics Aminohydrolases / metabolism* Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Endoplasmic Reticulum / enzymology Escherichia coli / enzymology* Escherichia coli / genetics Escherichia coli Proteins / chemistry Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Glycine / analogs & derivatives* Glycine / metabolism Hydrolysis Manganese / metabolism* Molecular Sequence Data Nitrogen / metabolism* Protein Structure, Secondary Urea / analogs & derivatives* Urea / metabolism Ureohydrolases / metabolism* |
| IF | 4.434 |
| 引用数 | 33 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | NA |