| RRC ID |
35287
|
| Author |
Soboh B, Krüger S, Kuhns M, Pinske C, Lehmann A, Sawers RG.
|
| Title |
Development of a cell-free system reveals an oxygen-labile step in the maturation of [NiFe]-hydrogenase 2 of Escherichia coli.
|
| Journal |
FEBS Lett
|
| Abstract |
By combining extracts from strains lacking genes encoding either the maturation enzymes or the large subunits of hydrogenases 1, 2 and 3 we could reconstitute in vitro under strictly anaerobic conditions 10-15% of the hydrogenase activity present in wild type Escherichia coli extracts. Purified, unprocessed Strep-tagged variants of the hydrogenase 2 large subunit, HybC, isolated from either a ΔhybD (encoding the hydrogenase 2-specific protease) mutant or a strain deficient in HypF could also be matured to active, processed enzyme using this system. These studies reveal that minimally one step early on the hydrogenase maturation pathway is oxygen-labile.
|
| Volume |
584(18)
|
| Pages |
4109-14
|
| Published |
2010-9-24
|
| DOI |
10.1016/j.febslet.2010.08.037
|
| PII |
S0014-5793(10)00699-X
|
| PMID |
20807532
|
| MeSH |
Carboxyl and Carbamoyl Transferases / genetics
Endopeptidases / genetics
Escherichia coli / enzymology*
Escherichia coli / genetics
Escherichia coli Proteins / chemistry
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism*
Hydrogenase / chemistry
Hydrogenase / genetics
Hydrogenase / metabolism*
Oxygen / metabolism*
|
| IF |
3.057
|
| Times Cited |
10
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| Prokaryotes E. coli |
JW2961
pCA24N-hybD? |
