RRC ID 35290
Author Takashima E, Yamada H, Yamashita T, Matsushita K, Konishi K.
Title Recombinant expression and redox properties of triheme c membrane-bound quinol peroxidase.
Journal FEMS Microbiol. Lett.
Abstract The qpo gene of Aggregatibacter actinomycetemcomitans encodes a triheme c-containing membrane-bound enzyme, quinol peroxidase (QPO) that catalyzes peroxidation reaction in the respiratory chain and uses quinol as the physiological electron donor. The QPO of A. actinomycetemcomitans is the only characterized QPO, but homologues of the qpo gene are widely distributed among many gram-negative bacteria, including Haemophils ducreii, Bacteroides fragilis, and Escherichia coli. One-third of the amino acid sequence of QPO from the N-terminal end is unique, whereas two-thirds of the sequence from the C-terminal end exhibits high homology with the sequence of the diheme bacterial cytochrome c peroxidase. In order to obtain sufficient protein for biophysical studies, the present study aimed to overproduce recombinant QPO (rQPO) from A. actinomycetemcomitans in E. coli. Coexpression of qpo with E. coli cytochrome c maturation (ccm) genes resulted in the expression of an active QPO with a high yield. Using purified rQPO, we determined the midpoint reduction potentials of the three heme molecules.
Volume 302(1)
Pages 52-7
Published 2010-1
DOI 10.1111/j.1574-6968.2009.01830.x
PMID 19895641
MeSH Antioxidants / chemistry Antioxidants / metabolism Bacterial Outer Membrane Proteins / biosynthesis Bacterial Outer Membrane Proteins / chemistry Bacterial Outer Membrane Proteins / genetics Escherichia coli / genetics Escherichia coli / metabolism Escherichia coli Proteins / biosynthesis Escherichia coli Proteins / genetics Heme / chemistry Heme / metabolism Humans Hydroquinones / chemistry Hydroquinones / metabolism Kinetics Oxidation-Reduction Pasteurellaceae / enzymology* Pasteurellaceae / genetics Pasteurellaceae Infections / complications Pasteurellaceae Infections / microbiology Periodontitis / etiology Peroxidases / biosynthesis* Peroxidases / chemistry Peroxidases / genetics Recombinant Proteins / biosynthesis* Recombinant Proteins / chemistry* Recombinant Proteins / genetics
IF 1.735
Times Cited 3
Prokaryotes E. coli NA