論文 - 詳細
| RRC ID | 35294 |
|---|---|
| 著者 | Wang C, Yoon SH, Shah AA, Chung YR, Kim JY, Choi ES, Keasling JD, Kim SW. |
| タイトル | Farnesol production from Escherichia coli by harnessing the exogenous mevalonate pathway. |
| ジャーナル | Biotechnol Bioeng |
| Abstract |
Farnesol (FOH) production has been carried out in metabolically engineered Escherichia coli. FOH is formed through the depyrophosphorylation of farnesyl pyrophosphate (FPP), which is synthesized from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) by FPP synthase. In order to increase FPP synthesis, E. coli was metabolically engineered to overexpress ispA and to utilize the foreign mevalonate (MVA) pathway for the efficient synthesis of IPP and DMAPP. Two-phase culture using a decane overlay of the culture broth was applied to reduce volatile loss of FOH produced during culture and to extract FOH from the culture broth. A FOH production of 135.5 mg/L was obtained from the recombinant E. coli harboring the pTispA and pSNA plasmids for ispA overexpression and MVA pathway utilization, respectively. It is interesting to observe that a large amount of FOH could be produced from E. coli without FOH synthase by the augmentation of FPP synthesis. Introduction of the exogenous MVA pathway enabled the dramatic production of FOH by E. coli while no detectable FOH production was observed in the endogenous MEP pathway-only control. |
| 巻・号 | 107(3) |
| ページ | 421-9 |
| 公開日 | 2010-10-15 |
| DOI | 10.1002/bit.22831 |
| PMID | 20552672 |
| MeSH | Culture Media / chemistry Escherichia coli / genetics* Escherichia coli / metabolism* Farnesol / metabolism* Gene Dosage Gene Expression Geranyltranstransferase / genetics Hemiterpenes / metabolism Metabolic Networks and Pathways / genetics* Mevalonic Acid / metabolism* Organophosphorus Compounds / metabolism Plasmids Polyisoprenyl Phosphates / metabolism Sesquiterpenes / metabolism |
| IF | 4.002 |
| 引用数 | 72 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME9062(BW25113)(Keio) JW0374-KC JW4015-KC JW3029-KC JW0408-KC JW1270-KC |