RRC ID 35294
Author Wang C, Yoon SH, Shah AA, Chung YR, Kim JY, Choi ES, Keasling JD, Kim SW.
Title Farnesol production from Escherichia coli by harnessing the exogenous mevalonate pathway.
Journal Biotechnol Bioeng
Abstract Farnesol (FOH) production has been carried out in metabolically engineered Escherichia coli. FOH is formed through the depyrophosphorylation of farnesyl pyrophosphate (FPP), which is synthesized from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) by FPP synthase. In order to increase FPP synthesis, E. coli was metabolically engineered to overexpress ispA and to utilize the foreign mevalonate (MVA) pathway for the efficient synthesis of IPP and DMAPP. Two-phase culture using a decane overlay of the culture broth was applied to reduce volatile loss of FOH produced during culture and to extract FOH from the culture broth. A FOH production of 135.5 mg/L was obtained from the recombinant E. coli harboring the pTispA and pSNA plasmids for ispA overexpression and MVA pathway utilization, respectively. It is interesting to observe that a large amount of FOH could be produced from E. coli without FOH synthase by the augmentation of FPP synthesis. Introduction of the exogenous MVA pathway enabled the dramatic production of FOH by E. coli while no detectable FOH production was observed in the endogenous MEP pathway-only control.
Volume 107(3)
Pages 421-9
Published 2010-10-15
DOI 10.1002/bit.22831
PMID 20552672
MeSH Culture Media / chemistry Escherichia coli / genetics* Escherichia coli / metabolism* Farnesol / metabolism* Gene Dosage Gene Expression Geranyltranstransferase / genetics Hemiterpenes / metabolism Metabolic Networks and Pathways / genetics* Mevalonic Acid / metabolism* Organophosphorus Compounds / metabolism Plasmids Polyisoprenyl Phosphates / metabolism Sesquiterpenes / metabolism
IF 4.002
Times Cited 72
Prokaryotes E. coli ME9062(BW25113)(Keio) JW0374-KC JW4015-KC JW3029-KC JW0408-KC JW1270-KC