論文 - 詳細
| RRC ID | 35296 |
|---|---|
| 著者 | Yano H, Miyakoshi M, Ohshima K, Tabata M, Nagata Y, Hattori M, Tsuda M. |
| タイトル | Complete nucleotide sequence of TOL plasmid pDK1 provides evidence for evolutionary history of IncP-7 catabolic plasmids. |
| ジャーナル | J Bacteriol |
| Abstract |
To understand the mechanisms for structural diversification of Pseudomonas-derived toluene-catabolic (TOL) plasmids, the complete sequence of a self-transmissible plasmid pDK1 with a size of 128,921 bp from Pseudomonas putida HS1 was determined. Comparative analysis revealed that (i) pDK1 consisted of a 75.6-kb IncP-7 plasmid backbone and 53.2-kb accessory gene segments that were bounded by transposon-associated regions, (ii) the genes for conjugative transfer of pDK1 were highly similar to those of MOB(H) group of mobilizable plasmids, and (iii) the toluene-catabolic (xyl) gene clusters of pDK1 were derived through homologous recombination, transposition, and site-specific recombination from the xyl gene clusters homologous to another TOL plasmid, pWW53. The minireplicons of pDK1 and its related IncP-7 plasmids, pWW53 and pCAR1, that contain replication and partition genes were maintained in all of six Pseudomonas strains tested, but not in alpha- or betaproteobacterial strains. The recipient host range of conjugative transfer of pDK1 was, however, limited to two Pseudomonas strains. These results indicate that IncP-7 plasmids are essentially narrow-host-range and self-transmissible plasmids that encode MOB(H) group-related transfer functions and that the host range of IncP-7-specified conjugative transfer was, unlike the situation in other well-known plasmids, narrower than that of its replication. |
| 巻・号 | 192(17) |
| ページ | 4337-47 |
| 公開日 | 2010-9-1 |
| DOI | 10.1128/JB.00359-10 |
| PII | JB.00359-10 |
| PMID | 20581207 |
| PMC | PMC2937381 |
| MeSH | Bacterial Proteins / genetics Conjugation, Genetic DNA Transposable Elements / genetics Evolution, Molecular* Molecular Sequence Data Multigene Family Plasmids / genetics* Pseudomonas putida / genetics* Pseudomonas putida / metabolism Recombination, Genetic Sequence Analysis, DNA Toluene / metabolism* |
| IF | 3.006 |
| 引用数 | 32 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 原核生物(大腸菌) | pJP5608 |