論文 - 詳細
| RRC ID | 35308 |
|---|---|
| 著者 | Storvik KA, Foster PL. |
| タイトル | RpoS, the stress response sigma factor, plays a dual role in the regulation of Escherichia coli's error-prone DNA polymerase IV. |
| ジャーナル | J Bacteriol |
| Abstract |
RpoS, Escherichia coli's general stress response sigma factor, regulates error-prone DNA polymerase IV (Pol IV) (encoded by the dinB gene). Pol IV is induced in stationary-phase cells, and thereafter, levels of the protein remain elevated for several days of continuous incubation. This induction and persistence in stationary-phase cells are dependent on RpoS. Data presented here show that this regulation is direct via the RpoS-directed transcription of the dinB gene. However, a loss of RpoS also results in a decrease in Pol IV-dependent mutation when Pol IV is overexpressed from an RpoS-independent promoter in exponentially growing cells. The loss of RpoS also increases cell sensitivity to 4-nitroquinoline-1-oxide, indicating that RpoS affects the ability of Pol IV to bypass DNA lesions. Thus, in addition to directly driving the transcription of the dinB gene in stationary-phase cells, RpoS regulates the activity of Pol IV in exponentially growing cells via a second, indirect pathway. |
| 巻・号 | 192(14) |
| ページ | 3639-44 |
| 公開日 | 2010-7-1 |
| DOI | 10.1128/JB.00358-10 |
| PII | JB.00358-10 |
| PMID | 20472798 |
| PMC | PMC2897332 |
| MeSH | Bacterial Proteins / genetics Bacterial Proteins / metabolism* Cell Division Escherichia coli / enzymology* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Deletion Gene Expression Regulation, Bacterial / physiology Gene Expression Regulation, Enzymologic / physiology* Sigma Factor / genetics Sigma Factor / metabolism* Transcription, Genetic |
| IF | 3.006 |
| 引用数 | 21 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | |