RRC ID 35313
著者 Teramoto J, Yoshimura SH, Takeyasu K, Ishihama A.
タイトル A novel nucleoid protein of Escherichia coli induced under anaerobiotic growth conditions.
ジャーナル Nucleic Acids Res
Abstract A systematic search was performed for DNA-binding sequences of YgiP, an uncharacterized transcription factor of Escherichia coli, by using the Genomic SELEX. A total of 688 YgiP-binding loci were identified after genome-wide profiling of SELEX fragments with a high-density microarray (SELEX-chip). Gel shift and DNase-I footprinting assays indicated that YgiP binds to multiple sites along DNA probes with a consensus GTTNATT sequence. Atomic force microscope observation indicated that at low concentrations, YgiP associates at various sites on DNA probes, but at high concentrations, YgiP covers the entire DNA surface supposedly through protein-protein contact. The intracellular concentration of YgiP is very low in growing E. coli cells under aerobic conditions, but increases more than 100-fold to the level as high as the major nucleoid proteins under anaerobic conditions. An E. coli mutant lacking ygiP showed retarded growth under anaerobic conditions. High abundance and large number of binding sites together indicate that YgiP is a nucleoid-associated protein with both architectural and regulatory roles as the nucleoid proteins Fis and IHF. We then propose that YgiP is a novel nucleoid protein of E. coli under anaerobiosis and propose to rename it Dan (DNA-binding protein under anaerobic conditions).
巻・号 38(11)
ページ 3605-18
公開日 2010-6-1
DOI 10.1093/nar/gkq077
PII gkq077
PMID 20156994
PMC PMC2887951
MeSH Anaerobiosis Base Sequence Binding Sites DNA Footprinting DNA, Bacterial / chemistry DNA, Bacterial / metabolism DNA, Bacterial / ultrastructure DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* DNA-Binding Proteins / ultrastructure Electrophoretic Mobility Shift Assay Escherichia coli / genetics Escherichia coli / growth & development Escherichia coli / metabolism Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Escherichia coli Proteins / ultrastructure Genomics Microscopy, Atomic Force Molecular Sequence Data SELEX Aptamer Technique Transcription Factors / genetics Transcription Factors / metabolism* Transcription Factors / ultrastructure
IF 11.502
引用数 39
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) JD24074