論文 - 詳細
| RRC ID | 35315 |
|---|---|
| 著者 | Vecerek B, Beich-Frandsen M, Resch A, Bläsi U. |
| タイトル | Translational activation of rpoS mRNA by the non-coding RNA DsrA and Hfq does not require ribosome binding. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
At low temperature, translational activation of rpoS mRNA, encoding the stationary phase sigma-factor, sigma(S), involves the small regulatory RNA (sRNA) DsrA and the RNA chaperone Hfq. The Hfq-mediated DsrA-rpoS interaction relieves an intramolecular secondary structure that impedes ribosome access to the rpoS ribosome binding site. In addition, DsrA/rpoS duplex formation creates an RNase III cleavage site within the duplex. Previous biochemical studies suggested that DsrA and Hfq associate with the 30S ribosomal subunit protein S1, which implied a role for the ribosome in sRNA-mediated post-transcriptional regulation. Here, we show by ribosome profiling that Hfq partitions with the cytoplasmic fraction rather than with 30S subunits. Besides, by employing immunological techniques, no evidence for a physical interaction between Hfq and S1 was obtained. Similarly, in vitro studies did not reveal a direct interaction between DsrA and S1. By employing a ribosome binding deficient rpoS mRNA, and by using the RNase III clevage in the DsrA/rpoS duplex as a diagnostic marker, we provide in vivo evidence that the Hfq-mediated DsrA/rpoS interaction, and consequently the structural changes in rpoS mRNA precede ribosome binding. These data suggest a simple mechanistic model in which translational activation by DsrA provides a translationally competent rpoS mRNA to which 30S subunits can readily bind. |
| 巻・号 | 38(4) |
| ページ | 1284-93 |
| 公開日 | 2010-3-1 |
| DOI | 10.1093/nar/gkp1125 |
| PII | gkp1125 |
| PMID | 19969548 |
| PMC | PMC2831331 |
| MeSH | Bacterial Proteins / biosynthesis Bacterial Proteins / genetics* Blotting, Western Cytoplasm / chemistry Escherichia coli / genetics Escherichia coli / metabolism Escherichia coli Proteins / analysis Escherichia coli Proteins / biosynthesis Escherichia coli Proteins / genetics* Escherichia coli Proteins / metabolism Gene Expression Regulation, Bacterial* Host Factor 1 Protein / analysis Host Factor 1 Protein / metabolism* Protein Biosynthesis* RNA, Messenger / chemistry RNA, Small Untranslated RNA, Untranslated / metabolism* Ribosomal Proteins / metabolism Ribosome Subunits, Small, Bacterial / metabolism Ribosomes / metabolism Sigma Factor / biosynthesis Sigma Factor / genetics* |
| IF | 11.502 |
| 引用数 | 30 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | JW4130-KC JW5437-KC |