| RRC ID |
35349
|
| Author |
Chadani Y, Ono K, Kutsukake K, Abo T.
|
| Title |
Escherichia coli YaeJ protein mediates a novel ribosome-rescue pathway distinct from SsrA- and ArfA-mediated pathways.
|
| Journal |
Mol Microbiol
|
| Abstract |
Accumulation of stalled ribosomes at the 3' end of mRNA without a stop codon (non-stop mRNA) is supposed to be toxic to bacterial cells. Escherichia coli has at least two distinct systems to rescue such stalled ribosomes: SsrA-dependent trans-translation and ArfA-dependent ribosome rescue. Combination of the ssrA and arfA mutations is synthetically lethal, suggesting the significance of ribosome rescue. In this study, we identified the E. coli yaeJ gene, encoding a peptide-release factor homologue with GGQ motif, as a multicopy suppressor of the lethal phenotype of ssrA arfA double mutant. The YaeJ protein was shown to bind to ribosomes. Both in vivo and in vitro, YaeJ showed the ribosome-rescue activity and promoted the hydrolysis of peptidyl-tRNA residing in the stalled ribosome. Missense mutation in the GGQ motif or deletion of the C-terminal unstructured tail abolished both the suppressor activity for ssrA arfA synthetic lethality and the ribosome-rescue activity, suggesting the importance of these structural features. On the basis of these observations, we propose that YaeJ acts as a stop codon-independent peptidyl-tRNA hydrolysing factor through binding to ribosomes stalled at the 3' end of non-stop mRNAs. It was also suggested that ArfA and YaeJ rescue the stalled ribosomes by distinct mechanisms.
|
| Volume |
80(3)
|
| Pages |
772-85
|
| Published |
2011-5-1
|
| DOI |
10.1111/j.1365-2958.2011.07607.x
|
| PMID |
21418110
|
| MeSH |
Amino Acid Sequence
Amino Acid Substitution / genetics
Carboxylic Ester Hydrolases / genetics
Carboxylic Ester Hydrolases / metabolism*
Escherichia coli / metabolism*
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism*
Gene Dosage
Molecular Sequence Data
Mutant Proteins / genetics
Mutant Proteins / metabolism
Mutation, Missense
Protein Binding
RNA, Transfer, Amino Acyl / metabolism
RNA-Binding Proteins / genetics
RNA-Binding Proteins / metabolism
Ribosomes / metabolism*
Sequence Alignment
Sequence Deletion
|
| IF |
3.418
|
| Times Cited |
76
|
|
WOS Category
|
MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Prokaryotes E. coli |
JW0187-KC |
