論文 - 詳細
| RRC ID | 35353 |
|---|---|
| 著者 | Eguchi Y, Ishii E, Hata K, Utsumi R. |
| タイトル | Regulation of acid resistance by connectors of two-component signal transduction systems in Escherichia coli. |
| ジャーナル | J Bacteriol |
| Abstract |
Two-component signal transduction systems (TCSs), utilized extensively by bacteria and archaea, are involved in the rapid adaptation of the organisms to fluctuating environments. A typical TCS transduces the signal by a phosphorelay between the sensor histidine kinase and its cognate response regulator. Recently, small-sized proteins that link TCSs have been reported and are called "connectors." Their physiological roles, however, have remained elusive. SafA (sensor associating factor A) (formerly B1500), a small (65-amino-acid [65-aa]) membrane protein, is among such connectors and links Escherichia coli TCSs EvgS/EvgA and PhoQ/PhoP. Since the activation of the EvgS/EvgA system induces acid resistance, we examined whether the SafA-activated PhoQ/PhoP system is also involved in the acid resistance induced by EvgS/EvgA. Using a constitutively active evgS1 mutant for the activation of EvgS/EvgA, we found that SafA, PhoQ, and PhoP all contributed to the acid resistance phenotype. Moreover, EvgS/EvgA activation resulted in the accumulation of cellular RpoS in the exponential-phase cells in a SafA-, PhoQ-, and PhoP-dependent manner. This RpoS accumulation was caused by another connector, IraM, expression of which was induced by the activation of the PhoQ/PhoP system, thus preventing RpoS degradation by trapping response regulator RssB. Acid resistance assays demonstrated that IraM also participated in the EvgS/EvgA-induced acid resistance. Therefore, we propose a model of a signal transduction cascade proceeding from EvgS/EvgA to PhoQ/PhoP and then to RssB (connected by SafA and IraM) and discuss its contribution to the acid resistance phenotype. |
| 巻・号 | 193(5) |
| ページ | 1222-8 |
| 公開日 | 2011-3-1 |
| DOI | 10.1128/JB.01124-10 |
| PII | JB.01124-10 |
| PMID | 21193607 |
| PMC | PMC3067605 |
| MeSH | Acids / pharmacology* Drug Resistance, Bacterial / genetics Drug Resistance, Bacterial / physiology Escherichia coli / drug effects* Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism Gene Deletion Gene Expression Regulation, Bacterial / physiology* Hydrogen-Ion Concentration Signal Transduction / physiology* |
| IF | 3.006 |
| 引用数 | 33 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 原核生物(大腸菌) | JW1115-KC JW1116-KC JW1147-KC |