RRC ID 35359
著者 Handa Y, Inaho N, Nameki N.
タイトル YaeJ is a novel ribosome-associated protein in Escherichia coli that can hydrolyze peptidyl-tRNA on stalled ribosomes.
ジャーナル Nucleic Acids Res.
Abstract In bacteria, ribosomes often become stalled and are released by a trans-translation process mediated by transfer-messenger RNA (tmRNA). In the absence of tmRNA, however, there is evidence that stalled ribosomes are released from non-stop mRNAs. Here, we show a novel ribosome rescue system mediated by a small basic protein, YaeJ, from Escherichia coli, which is similar in sequence and structure to the catalytic domain 3 of polypeptide chain release factor (RF). In vitro translation experiments using the E. coli-based reconstituted cell-free protein synthesis system revealed that YaeJ can hydrolyze peptidyl-tRNA on ribosomes stalled by both non-stop mRNAs and mRNAs containing rare codon clusters that extend downstream from the P-site and prevent Ala-tmRNA•SmpB from entering the empty A-site. In addition, YaeJ had no effect on translation of a normal mRNA with a stop codon. These results suggested a novel tmRNA-independent rescue system for stalled ribosomes in E. coli. YaeJ was almost exclusively found in the 70S ribosome and polysome fractions after sucrose density gradient sedimentation, but was virtually undetectable in soluble fractions. The C-terminal basic residue-rich extension was also found to be required for ribosome binding. These findings suggest that YaeJ functions as a ribosome-attached rescue device for stalled ribosomes.
巻・号 39(5)
ページ 1739-48
公開日 2010-11-6
DOI 10.1093/nar/gkq1097
PII gkq1097
PMID 21051357
PMC PMC3061065
MeSH Amino Acid Motifs Carboxylic Ester Hydrolases / chemistry Carboxylic Ester Hydrolases / metabolism* Codon Escherichia coli / enzymology* Escherichia coli / genetics Escherichia coli Proteins / chemistry Escherichia coli Proteins / metabolism* Hydrolysis Protein Binding RNA, Transfer, Amino Acyl / metabolism* Ribosomal Proteins / chemistry Ribosomal Proteins / metabolism* Ribosomes / metabolism*
IF 10.162
引用数 47
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) MG1655? JW0187-KC ME9062(BW25113)