論文 - 詳細
| RRC ID | 35362 |
|---|---|
| 著者 | Landry AP, Duan X, Huang H, Ding H. |
| タイトル | Iron-sulfur proteins are the major source of protein-bound dinitrosyl iron complexes formed in Escherichia coli cells under nitric oxide stress. |
| ジャーナル | Free Radic Biol Med |
| Abstract |
Protein-bound dinitrosyl iron complexes (DNICs) have been observed in prokaryotic and eukaryotic cells under nitric oxide (NO) stress. The identity of proteins that bind DNICs, however, still remains elusive. Here we demonstrate that iron-sulfur proteins are the major source of protein-bound DNICs formed in Escherichia coli cells under NO stress. Expression of recombinant iron-sulfur proteins, but not proteins without iron-sulfur clusters, almost doubles the amount of protein-bound DNICs formed in E. coli cells after NO exposure. Purification of recombinant proteins from the NO-exposed E. coli cells further confirms that iron-sulfur proteins, but not proteins without iron-sulfur clusters, are modified, forming protein-bound DNICs. Deletion of the iron-sulfur cluster assembly proteins IscA and SufA to block the [4Fe-4S] cluster biogenesis in E. coli cells largely eliminates the NO-mediated formation of protein-bound DNICs, suggesting that iron-sulfur clusters are mainly responsible for the NO-mediated formation of protein-bound DNICs in cells. Furthermore, depletion of the "chelatable iron pool" in wild-type E. coli cells effectively removes iron-sulfur clusters from proteins and concomitantly diminishes the NO-mediated formation of protein-bound DNICs, indicating that iron-sulfur clusters in proteins constitute at least part of the chelatable iron pool in cells. |
| 巻・号 | 50(11) |
| ページ | 1582-90 |
| 公開日 | 2011-6-1 |
| DOI | 10.1016/j.freeradbiomed.2011.03.005 |
| PII | S0891-5849(11)00162-6 |
| PMID | 21420489 |
| PMC | PMC3090472 |
| MeSH | Cloning, Molecular Electron Spin Resonance Spectroscopy Escherichia coli / physiology* Escherichia coli Proteins / chemistry Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Iron / metabolism* Iron Chelating Agents / chemistry Iron Chelating Agents / metabolism* Iron-Sulfur Proteins / chemistry Iron-Sulfur Proteins / genetics Iron-Sulfur Proteins / metabolism* Nitric Oxide / metabolism Nitrogen Oxides / metabolism* Oxidative Stress Protein Binding Protein Processing, Post-Translational Recombinant Proteins / genetics |
| IF | 6.17 |
| 引用数 | 27 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 原核生物(大腸菌) | JW5605(ASKA) JW4024(ASKA) JW2510(ASKA) JW4020(ASKA) |