RRC ID 35363
Author Marques JC, Lamosa P, Russell C, Ventura R, Maycock C, Semmelhack MF, Miller ST, Xavier KB.
Title Processing the interspecies quorum-sensing signal autoinducer-2 (AI-2): characterization of phospho-(S)-4,5-dihydroxy-2,3-pentanedione isomerization by LsrG protein.
Journal J. Biol. Chem.
Abstract The molecule (S)-4,5-dihydroxy-2,3-pentanedione (DPD) is produced by many different species of bacteria and is the precursor of the signal molecule autoinducer-2 (AI-2). AI-2 mediates interspecies communication and facilitates regulation of bacterial behaviors such as biofilm formation and virulence. A variety of bacterial species have the ability to sequester and process the AI-2 present in their environment, thereby interfering with the cell-cell communication of other bacteria. This process involves the AI-2-regulated lsr operon, comprised of the Lsr transport system that facilitates uptake of the signal, a kinase that phosphorylates the signal to phospho-DPD (P-DPD), and enzymes (like LsrG) that are responsible for processing the phosphorylated signal. Because P-DPD is the intracellular inducer of the lsr operon, enzymes involved in P-DPD processing impact the levels of Lsr expression. Here we show that LsrG catalyzes isomerization of P-DPD into 3,4,4-trihydroxy-2-pentanone-5-phosphate. We present the crystal structure of LsrG, identify potential catalytic residues, and determine which of these residues affects P-DPD processing in vivo and in vitro. We also show that an lsrG deletion mutant accumulates at least 10 times more P-DPD than wild type cells. Consistent with this result, we find that the lsrG mutant has increased expression of the lsr operon and an altered profile of AI-2 accumulation and removal. Understanding of the biochemical mechanisms employed by bacteria to quench signaling of other species can be of great utility in the development of therapies to control bacterial behavior.
Volume 286(20)
Pages 18331-43
Published 2011-5-20
DOI 10.1074/jbc.M111.230227
PII M111.230227
PMID 21454635
PMC PMC3093905
MeSH Crystallography, X-Ray Escherichia coli / enzymology* Escherichia coli / genetics Escherichia coli Proteins* / chemistry Escherichia coli Proteins* / genetics Escherichia coli Proteins* / metabolism Gene Deletion Homoserine / analogs & derivatives* Homoserine / chemistry Homoserine / metabolism Lactones* / chemistry Lactones* / metabolism Mixed Function Oxygenases* / chemistry Mixed Function Oxygenases* / genetics Mixed Function Oxygenases* / metabolism Operon / physiology Pentanones* / chemistry Pentanones* / metabolism Protein Structure, Tertiary Quorum Sensing / physiology*
IF 4.011
Times Cited 25
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Prokaryotes E. coli JW1504-KC