RRC ID 35384
著者 Haverkorn van Rijsewijk BR, Nanchen A, Nallet S, Kleijn RJ, Sauer U.
タイトル Large-scale 13C-flux analysis reveals distinct transcriptional control of respiratory and fermentative metabolism in Escherichia coli.
ジャーナル Mol Syst Biol
Abstract Despite our increasing topological knowledge on regulation networks in model bacteria, it is largely unknown which of the many co-occurring regulatory events actually control metabolic function and the distribution of intracellular fluxes. Here, we unravel condition-dependent transcriptional control of Escherichia coli metabolism by large-scale (13)C-flux analysis in 91 transcriptional regulator mutants on glucose and galactose. In contrast to the canonical respiro-fermentative glucose metabolism, fully respiratory galactose metabolism depends exclusively on the phosphoenol-pyruvate (PEP)-glyoxylate cycle. While 2/3 of the regulators directly or indirectly affected absolute flux rates, the partitioning between different pathways remained largely stable with transcriptional control focusing primarily on the acetyl-CoA branch point. Flux distribution control was achieved by nine transcription factors on glucose, including ArcA, Fur, PdhR, IHF A and IHF B, but was exclusively mediated by the cAMP-dependent Crp regulation of the PEP-glyoxylate cycle flux on galactose. Five further transcription factors affected this flux only indirectly through cAMP and Crp by increasing the galactose uptake rate. Thus, E. coli actively limits its galactose catabolism at the expense of otherwise possible faster growth.
巻・号 7
ページ 477
公開日 2011-3-29
DOI 10.1038/msb.2011.9
PII msb20119
PMID 21451587
PMC PMC3094070
MeSH Acetyl Coenzyme A / metabolism* Carbon / metabolism* Carbon Isotopes / metabolism Citric Acid Cycle Cyclic AMP / metabolism Escherichia coli / genetics Escherichia coli / growth & development Escherichia coli / metabolism Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism Galactose / metabolism* Gene Expression Regulation, Bacterial* Glucose / metabolism* Glycolysis Glyoxylates / metabolism Isotope Labeling Oxygen / metabolism Phosphoenolpyruvate / metabolism Transcription Factors / genetics Transcription Factors / metabolism Transcription, Genetic
IF 8.991
引用数 100
リソース情報
原核生物(大腸菌) Keio collection