| RRC ID |
35386
|
| Author |
Sobue R, Kurono N, Etchuya R, Maeda S.
|
| Title |
Identification of a novel DNA element that promotes cell-to-cell transformation in Escherichia coli.
|
| Journal |
FEBS Lett
|
| Abstract |
Recently, we discovered a novel phenomenon, "cell-to-cell transformation" by which non-conjugative plasmids are transmitted horizontally in co-cultures of Escherichia coli F(-) strains. In this study, we aimed to identify the DNA element responsible for the high cell-to-cell transformability of pHSG299. By transplanting pHSG299 DNA fragments into pHSG399, a plasmid showing low transformability, we discovered that a specific 88 bp fragment of pHSG299 significantly promoted pHSG399 transformability. Although several short motif-like repetitive sequences (6-10 bp) were present in the 88 bp sequence, no known DNA motifs were recognized, suggesting that this 88 bp sequence (cell-to-cell transformation promoting sequence, CTPS; Accession number: AB634455) is a novel DNA element.
|
| Volume |
585(14)
|
| Pages |
2223-8
|
| Published |
2011-7-21
|
| DOI |
10.1016/j.febslet.2011.05.040
|
| PII |
S0014-5793(11)00406-6
|
| PMID |
21621537
|
| MeSH |
Base Sequence
DNA Transposable Elements / genetics*
DNA, Bacterial / genetics*
Escherichia coli / genetics*
Molecular Sequence Data
Plasmids / genetics
Plasmids / metabolism
Transformation, Genetic*
|
| IF |
3.057
|
| Times Cited |
8
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| Prokaryotes E. coli |
ME9033(DH5)
ME8788(CAG18439)
pHSG299
pHSG399
pUC19 |
