RRC ID 35387
著者 Narita S, Tokuda H.
タイトル Overexpression of LolCDE allows deletion of the Escherichia coli gene encoding apolipoprotein N-acyltransferase.
ジャーナル J Bacteriol
Abstract Bacterial lipoproteins represent a subset of membrane-associated proteins that are covalently modified with lipids at the N-terminal cysteine. The final step of lipoprotein modification, N-acylation of apolipoproteins, is mediated by apolipoprotein N-acyltransferase (Lnt). Examinations with reconstituted proteoliposomes and a conditional mutant previously indicated that N-acylation of lipoproteins is required for their efficient release from the inner membrane catalyzed by LolA and LolCDE, the lipoprotein-specific chaperone and ABC transporter, respectively. Because Lnt is essential for Escherichia coli, a mutant lacking Lnt activity has not been isolated. However, we report here that lnt-null strains can be constructed when LolCDE is overproduced in strains lacking either the major outer membrane lipoprotein Lpp or transpeptidases that cross-link Lpp with peptidoglycan. Lipoproteins purified from the lnt-null strain exhibited increased mobility on SDS-PAGE compared to those from wild-type cells and could be sequenced by Edman degradation, indicating that lipoproteins in this mutant exist as apolipoproteins that lack N-acylation. Overexpression of Lpp in the lnt-null strain resulted in the accumulation of apoLpp in the inner membrane and caused growth arrest. In contrast to the release of mature Lpp in the presence of LolA and LolCDE, that of apoLpp from the inner membrane was significantly retarded. Furthermore, the amount of lipoproteins copurified with LolCDE was significantly reduced in the lnt-null strain. These results indicate that the affinity of LolCDE for apolipoprotein is very low, and therefore, overexpression of LolCDE is required for its release and sorting to the outer membrane.
巻・号 193(18)
ページ 4832-40
公開日 2011-9-1
DOI 10.1128/JB.05013-11
PII JB.05013-11
PMID 21742870
PMC PMC3165678
MeSH ATP-Binding Cassette Transporters / biosynthesis* ATP-Binding Cassette Transporters / genetics Acyltransferases / deficiency Acyltransferases / metabolism* Electrophoresis, Polyacrylamide Gel Electrophoretic Mobility Shift Assay Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / analysis Escherichia coli Proteins / biosynthesis* Escherichia coli Proteins / genetics Gene Deletion* Gene Expression* Lipoproteins / analysis
IF 3.006
引用数 18
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) JW1105-KC Keio collection pCP20?