RRC ID 35393
Author Mattenberger Y, Mattson S, Métrailler J, Silva F, Belin D.
Title 55.1, a gene of unknown function of phage T4, impacts on Escherichia coli folate metabolism and blocks DNA repair by the NER.
Journal Mol. Microbiol.
Abstract Phage T4, the archetype of lytic bacterial viruses, needs only 62 genes to propagate under standard laboratory conditions. Interestingly, the T4 genome contains more than 100 putative genes of unknown function, with few detectable homologues in cellular genomes. To characterize this uncharted territory of genetic information, we have identified several T4 genes that prevent bacterial growth when expressed from plasmids under inducible conditions. Here, we report on the various phenotypes and molecular characterization of 55.1, one of the genes of unknown function. High-level expression from the arabinose-inducible P(BAD) promoter is toxic to the bacteria and delays the intracellular accumulation of phage without affecting the final burst size. Low-level expression from T4 promoter(s) renders bacteria highly sensitive to UV irradiation and hypersensitive to trimethoprim, an inhibitor of dihydrofolate reductase. The delay in intracellular phage accumulation requires UvsW, a T4 helicase that is also a suppressor of 55.1-induced toxicity and UV sensitivity. Genetic and biochemical experiments demonstrate that gp55.1 binds to FolD, a key enzyme of the folate metabolism and suppressor of 55.1. Finally, we show that gp55.1 prevents the repair of UV-induced DNA photoproducts by the nucleotide excision repair (NER) pathway through interaction with the UvrA and UvrB proteins.
Volume 82(6)
Pages 1406-21
Published 2011-12
DOI 10.1111/j.1365-2958.2011.07897.x
PMID 22029793
MeSH Bacteriophage T4 / genetics* Bacteriophage T4 / metabolism DNA Damage / radiation effects DNA Repair* / radiation effects Escherichia coli / genetics* Escherichia coli / metabolism* Escherichia coli / radiation effects Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism Folic Acid / metabolism* Gene Expression* Ultraviolet Rays Viral Proteins / genetics Viral Proteins / metabolism*
IF 3.816
Times Cited 2
WOS Category MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Prokaryotes E. coli ?