論文 - 詳細
| RRC ID | 35403 |
|---|---|
| 著者 | Hsiao YY, Yang CC, Lin CL, Lin JL, Duh Y, Yuan HS. |
| タイトル | Structural basis for RNA trimming by RNase T in stable RNA 3'-end maturation. |
| ジャーナル | Nat Chem Biol |
| Abstract |
RNA maturation relies on various exonucleases to remove nucleotides successively from the 5' or 3' end of nucleic acids. However, little is known regarding the molecular basis for substrate and cleavage preference of exonucleases. Our biochemical and structural analyses on RNase T-DNA complexes show that the RNase T dimer has an ideal architecture for binding a duplex with a short 3' overhang to produce a digestion product of a duplex with a 2-nucleotide (nt) or 1-nt 3' overhang, depending on the composition of the last base pair in the duplex. A 'C-filter' in RNase T screens out the nucleic acids with 3'-terminal cytosines for hydrolysis by inducing a disruptive conformational change at the active site. Our results reveal the general principles and the working mechanism for the final trimming step made by RNase T in the maturation of stable RNA and pave the way for the understanding of other DEDD family exonucleases. |
| 巻・号 | 7(4) |
| ページ | 236-43 |
| 公開日 | 2011-4-1 |
| DOI | 10.1038/nchembio.524 |
| PII | nchembio.524 |
| PMID | 21317904 |
| MeSH | 3' Untranslated Regions* Base Sequence Catalytic Domain Cytosine / chemistry Cytosine / metabolism Dimerization Escherichia coli / enzymology Escherichia coli / genetics Escherichia coli / metabolism* Exonucleases / metabolism Exoribonucleases / chemistry* Exoribonucleases / genetics Exoribonucleases / metabolism Hydrolysis Nucleic Acid Conformation RNA / chemistry* RNA / genetics RNA / metabolism Substrate Specificity |
| IF | 12.587 |
| 引用数 | 26 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | Keio collection |