RRC ID 35416
Author Delvillani F, Papiani G, Dehò G, Briani F.
Title S1 ribosomal protein and the interplay between translation and mRNA decay.
Journal Nucleic Acids Res
Abstract S1 is an 'atypical' ribosomal protein weakly associated with the 30S subunit that has been implicated in translation, transcription and control of RNA stability. S1 is thought to participate in translation initiation complex formation by assisting 30S positioning in the translation initiation region, but little is known about its role in other RNA transactions. In this work, we have analysed in vivo the effects of different intracellular S1 concentrations, from depletion to overexpression, on translation, decay and intracellular distribution of leadered and leaderless messenger RNAs (mRNAs). We show that the cspE mRNA, like the rpsO transcript, may be cleaved by RNase E at multiple sites, whereas the leaderless cspE transcript may also be degraded via an alternative pathway by an unknown endonuclease. Upon S1 overexpression, RNase E-dependent decay of both cspE and rpsO mRNAs is suppressed and these transcripts are stabilized, whereas cleavage of leaderless cspE mRNA by the unidentified endonuclease is not affected. Overall, our data suggest that ribosome-unbound S1 may inhibit translation and that part of the Escherichia coli ribosomes may actually lack S1.
Volume 39(17)
Pages 7702-15
Published 2011-9-1
DOI 10.1093/nar/gkr417
PII gkr417
PMID 21685451
PMC PMC3177188
MeSH 5' Untranslated Regions Endoribonucleases / antagonists & inhibitors Escherichia coli / genetics Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Heat-Shock Proteins / genetics Protein Biosynthesis* RNA Stability* RNA, Messenger / analysis RNA, Messenger / metabolism* Ribosomal Proteins / metabolism* Ribosomes / metabolism
IF 11.502
Times Cited 36
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Prokaryotes E. coli JW0618-KC JW3216-KC JW2263-KC