RRC ID 35419
Author Shimada T, Fujita N, Yamamoto K, Ishihama A.
Title Novel roles of cAMP receptor protein (CRP) in regulation of transport and metabolism of carbon sources.
Journal PLoS One
Abstract CRP (cAMP receptor protein), the global regulator of genes for carbon source utilization in the absence of glucose, is the best-studied prokaryotic transcription factor. A total of 195 target promoters on the Escherichia coli genome have been proposed to be under the control of cAMP-bound CRP. Using the newly developed Genomic SELEX screening system of transcription factor-binding sequences, however, we have identified a total of at least 254 CRP-binding sites. Based on their location on the E. coli genome, we predict a total of at least 183 novel regulation target operons, altogether with the 195 hitherto known targets, reaching to the minimum of 378 promoters as the regulation targets of cAMP-CRP. All the promoters selected from the newly identified targets and examined by using the lacZ reporter assay were found to be under the control of CRP, indicating that the Genomic SELEX screening allowed to identify the CRP targets with high accuracy. Based on the functions of novel target genes, we conclude that CRP plays a key regulatory role in the whole processes from the selective transport of carbon sources, the glycolysis-gluconeogenesis switching to the metabolisms downstream of glycolysis, including tricarboxylic acid (TCA) cycle, pyruvate dehydrogenase (PDH) pathway and aerobic respiration. One unique regulation mode is that a single and the same CRP molecule bound within intergenic regions often regulates both of divergently transcribed operons.
Volume 6(6)
Pages e20081
Published 2011-1-1
DOI 10.1371/journal.pone.0020081
PII PONE-D-11-03781
PMID 21673794
PMC PMC3105977
MeSH Base Sequence Binding Sites Biological Transport Carbon / metabolism* Consensus Sequence Cyclic AMP / metabolism Cyclic AMP Receptor Protein / chemistry Cyclic AMP Receptor Protein / metabolism* Escherichia coli / genetics Escherichia coli / metabolism Escherichia coli Proteins / chemistry Escherichia coli Proteins / metabolism* Genomics Oligonucleotide Array Sequence Analysis Transcription, Genetic
IF 2.74
Times Cited 127
Prokaryotes E. coli ME9062(BW25113)(Keio) JW5702-KC