論文 - 詳細
| RRC ID | 35425 |
|---|---|
| 著者 | Ashok S, Raj SM, Rathnasingh C, Park S. |
| タイトル | Development of recombinant Klebsiella pneumoniae ∆dhaT strain for the co-production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol. |
| ジャーナル | Appl Microbiol Biotechnol |
| Abstract |
Klebsiella pneumoniae converts glycerol to the specialty chemical 1,3-propanediol (1,3-PDO), which is used for the production of polytrimethylene terepthalate (PTT). In this study, an NAD(+)-dependent gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase (PuuC) of K. pneumoniae DSM 2026, which oxidizes 3-hydroxypropionaldehyde to a platform chemical 3-hydroxypropionic acid (3-HP), was cloned and overexpressed in K. pneumoniae DSM 2026 for the co-production of 3-HP and 1,3-PDO from glycerol. In addition, the gene dhaT, encoding NADH-dependent 1,3-propanediol oxidoreductase (1,3-PDOR), was deleted from the chromosome for the balanced production of 3-HP and 1,3-PDO. The recombinant K. pneumoniae ∆dhaT, expressing puuC, produced 3.6 g 3-HP and 3.0 g 1,3-PDO per liter with an average yield of 81% on glycerol carbon in shake flask culture under microaerobic conditions. When a fed-batch culture was carried out under microaerobic conditions at pH 7.0 in a 5-l bioreactor, the recombinant K. pneumoniae ∆dhaT (puuC) strain produced 16.0 g 3-HP and 16.8 g 1,3-PDO per liter with a cumulative yield of 51% on glycerol carbon in 24 h. The production of 1,3-PDO in the dhaT-deletion mutant was attributed to the expression of NAD(P)H-dependent hypothetical oxidoreductase. This study demonstrates the feasibility of obtaining two commercially valuable chemicals, 3-HP and 1,3-PDO, at a significant scale. |
| 巻・号 | 90(4) |
| ページ | 1253-65 |
| 公開日 | 2011-5-1 |
| DOI | 10.1007/s00253-011-3148-z |
| PMID | 21336929 |
| MeSH | Alcohol Dehydrogenase / genetics* Alcohol Dehydrogenase / metabolism Aldehyde Oxidoreductases / genetics Aldehyde Oxidoreductases / metabolism Bacterial Proteins / genetics* Bacterial Proteins / metabolism Gene Deletion* Genetic Engineering Glycerol / metabolism* Klebsiella pneumoniae / enzymology Klebsiella pneumoniae / genetics Klebsiella pneumoniae / metabolism* Lactic Acid / analogs & derivatives* Lactic Acid / metabolism Propylene Glycols / metabolism* |
| IF | 3.53 |
| 引用数 | 76 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | |