論文 - 詳細
| RRC ID | 35440 |
|---|---|
| 著者 | Shimizu T, Tsutsuki H, Matsumoto A, Nakaya H, Noda M. |
| タイトル | The nitric oxide reductase of enterohaemorrhagic Escherichia coli plays an important role for the survival within macrophages. |
| ジャーナル | Mol Microbiol |
| Abstract |
In enterohaemorrhagic Escherichia coli (EHEC) O157, there are two types of anaerobic nitric oxide (NO) reductase genes, an intact gene (norV) and a 204 bp deletion gene (norVs). Epidemiological analysis has revealed that norV-type EHEC are more virulent than norVs-type EHEC. Thus, to reveal the role of NO reductase during EHEC infection, we constructed isogenic norV-type and norVs-type EHEC mutant strains. Under anaerobic conditions, the norV-type EHEC was protected from NO-mediated growth inhibition, while the norVs-type EHEC mutant strain was not, suggesting that NorV of EHEC was effective in the anaerobic detoxification. We then investigated the role of NO reductase within macrophages. The norV-type EHEC produced a lower NO level within macrophages compared with the norVs-type EHEC. Moreover, the norV-type EHEC resulted in higher levels of Shiga toxin 2 (Stx2) within macrophages compared with the norVs-type EHEC. Finally, the norV-type EHEC showed a better level of survival than the norVs-type EHEC. These data suggest that the intact norV gene plays an important role for the survival of EHEC within macrophages, and is a direct virulence determinant of EHEC. |
| 巻・号 | 85(3) |
| ページ | 492-512 |
| 公開日 | 2012-8-1 |
| DOI | 10.1111/j.1365-2958.2012.08122.x |
| PMID | 22716767 |
| MeSH | Anaerobiosis / genetics Escherichia coli O157 / genetics* Escherichia coli O157 / metabolism Escherichia coli O157 / pathogenicity* Gene Deletion Gene Expression Regulation, Bacterial Gene Order Macrophages / metabolism Macrophages / microbiology* Nitric Oxide / metabolism Operon / genetics Oxidoreductases / genetics* Oxidoreductases / metabolism Shiga Toxin 1 / biosynthesis Shiga Toxin 1 / genetics Virulence Factors / genetics* Virulence Factors / metabolism |
| IF | 3.418 |
| 引用数 | 39 |
| WOS 分野 | MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | pFT-A |
| ヒト・動物細胞 | RAW 264(RCB0535) |