RRC ID 35440
著者 Shimizu T, Tsutsuki H, Matsumoto A, Nakaya H, Noda M.
タイトル The nitric oxide reductase of enterohaemorrhagic Escherichia coli plays an important role for the survival within macrophages.
ジャーナル Mol Microbiol
Abstract In enterohaemorrhagic Escherichia coli (EHEC) O157, there are two types of anaerobic nitric oxide (NO) reductase genes, an intact gene (norV) and a 204 bp deletion gene (norVs). Epidemiological analysis has revealed that norV-type EHEC are more virulent than norVs-type EHEC. Thus, to reveal the role of NO reductase during EHEC infection, we constructed isogenic norV-type and norVs-type EHEC mutant strains. Under anaerobic conditions, the norV-type EHEC was protected from NO-mediated growth inhibition, while the norVs-type EHEC mutant strain was not, suggesting that NorV of EHEC was effective in the anaerobic detoxification. We then investigated the role of NO reductase within macrophages. The norV-type EHEC produced a lower NO level within macrophages compared with the norVs-type EHEC. Moreover, the norV-type EHEC resulted in higher levels of Shiga toxin 2 (Stx2) within macrophages compared with the norVs-type EHEC. Finally, the norV-type EHEC showed a better level of survival than the norVs-type EHEC. These data suggest that the intact norV gene plays an important role for the survival of EHEC within macrophages, and is a direct virulence determinant of EHEC.
巻・号 85(3)
ページ 492-512
公開日 2012-8-1
DOI 10.1111/j.1365-2958.2012.08122.x
PMID 22716767
MeSH Anaerobiosis / genetics Escherichia coli O157 / genetics* Escherichia coli O157 / metabolism Escherichia coli O157 / pathogenicity* Gene Deletion Gene Expression Regulation, Bacterial Gene Order Macrophages / metabolism Macrophages / microbiology* Nitric Oxide / metabolism Operon / genetics Oxidoreductases / genetics* Oxidoreductases / metabolism Shiga Toxin 1 / biosynthesis Shiga Toxin 1 / genetics Virulence Factors / genetics* Virulence Factors / metabolism
IF 3.418
引用数 39
WOS 分野 MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) pFT-A
ヒト・動物細胞 RAW 264(RCB0535)