RRC ID 35443
著者 Kurono N, Matsuda A, Etchuya R, Sobue R, Sasaki Y, Ito M, Ando T, Maeda S.
タイトル Genome-wide screening of Escherichia coli genes involved in execution and promotion of cell-to-cell transfer of non-conjugative plasmids: rodZ (yfgA) is essential for plasmid acceptance in recipient cells.
ジャーナル Biochem Biophys Res Commun
Abstract Acquisition of new genetic traits by horizontal gene transfer is a bacterial strategy for adaptation to the environment. We previously showed that Escherichia coli can transmit non-conjugative plasmids laterally in a co-culture containing strains with and without the plasmid. In this study, using the Keio collection, a comprehensive library of E. coli knock-out mutants for non-essential genes, we screened for genes responsible for the execution and promotion of cell-to-cell plasmid transfer in recipient cells. By stepwise screening of 'transfer-down' mutants, two essential genes and six promoting genes were obtained. One of the essential genes was priA, which is involved in DNA replication. This priA mutant was also unable to be transformed by artificial transformation methods, probably due to the deficiency of the plasmid maintenance function. The other essential gene was rodZ (yfgA), a gene involved in the regulation of rod-shaped structure of E. coli cells. This rodZ mutant was transformable by all three methods of artificial transformation tested, suggesting that this gene is essential for cell-to-cell plasmid transfer but not for artificial transformation. These are the first data that suggest that rodZ plays an essential role in DNA acquisition.
巻・号 421(1)
ページ 119-23
公開日 2012-4-27
DOI 10.1016/j.bbrc.2012.03.127
PII S0006-291X(12)00597-9
PMID 22497891
MeSH Conjugation, Genetic / genetics Cytoskeletal Proteins / genetics Cytoskeletal Proteins / physiology* DNA / genetics Escherichia coli / genetics* Escherichia coli Proteins / genetics Escherichia coli Proteins / physiology* Gene Transfer, Horizontal / genetics* Genome-Wide Association Study* Plasmids / genetics* Transformation, Bacterial / genetics*
IF 2.985
引用数 9
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) ME8788(CAG18439) ME9062(BW25113) Keio collection pSY510 pGBM1